生物启发介导的银纳米粒子在生物医学和光催化方面的应用。
Biomedical and Photo-Catalytic Application of Bio-Inspired Mediated Silver Nanoparticles.
出版信息
J Biomed Nanotechnol. 2020 Apr 1;16(4):492-504. doi: 10.1166/jbn.2020.2918.
To minimize the hazardous effect of physical and chemical synthesis of nanoparticles we focused on the green synthesis of nanoparticles. Nanotechnology is a research hotspot and catch great attention because of its versatile applications in medical, biosciences and engineering fields. Purpose of our recent study is to synthesize bio-inspired metallic silver NPs by root mediated extract. The synthesized Ag-NPs were further characterized by using UVVisible spectroscopy, XRD, EDX, SEM, TEM and DLS techniques. The extent of crystallites were confirmed by X-ray diffraction. SEM and TEM revealed the morphological features with size of nanoparticles between 17.3 and 41.2 nm. FTIR analysis confirmed the capping of nanoparticles by bio active constituents present in extract. Later EDX confirmed the elemental composition of nanoparticles. Zeta potential, PDI and hydrodynamic size of Ag-NPs were confirmed by DLS. The synthesize Ag-NPs possess eminent biological potency against bacterial and leishmanial strains. Moreover considerable anti-diabetic, anticancer, antioxidant and biocompatibility nature of Ag-NPs was elucidated. The highest antioxidant activity of 50.61± 1.12%, 38.22 ± 1.18% and 27.39 ± 0.92 at 200 g/mL for TAC, TRP DPPH and was observed respectively. Ag-NPs exhibit potent leishmanicidal activity of 80% ± 1.4 against promastigotes and 77% ± 1.6 against amastigotes cultures of . Highest antidiabetic activity 30 ± 0.77% recorded at 200 g/ml. Highest Brine shrimps cytotoxicity of Ag-NPs was 60 ± 1.18 at 200 g/ml. Maximum dye degradation for Ag-NPs was recorded as 94.1% at 140 minute. All UTI isolates were resistant to antibiotics not coated with Ag-NPs. By applying 1% of Ag-NPs highest activity was recorded as 25 ± 1.58 mm against . Maximum zone of inhibition for Ag-NPs coated with Imipenem antibiotics 26 ± 1.5 mm against and coated with Ciprofloxacin 26 ± 1.4 m against were measured. Last but not least high biocompatible nature of Ag-NPs was observed against fresh RBCs making the ecofriendly biosynthesized silver NPs a multi-dimensional candidate in biomedical field.
为了最大限度地减少物理和化学合成纳米粒子的危害性，我们专注于纳米粒子的绿色合成。纳米技术是一个研究热点，因其在医学、生物科学和工程领域的广泛应用而备受关注。我们最近的研究目的是通过根介导的提取物合成仿生金属银 NPs。合成的 Ag-NPs 进一步通过 UVVisible 光谱、XRD、EDX、SEM、TEM 和 DLS 技术进行了表征。通过 X 射线衍射证实了微晶的程度。SEM 和 TEM 显示了纳米粒子的形态特征，其粒径在 17.3 至 41.2nm 之间。FTIR 分析证实了生物活性成分对提取物中纳米粒子的包覆。随后 EDX 证实了纳米粒子的元素组成。通过 DLS 证实了 Ag-NPs 的 ζ 电位、PDI 和水动力粒径。合成的 Ag-NPs 对细菌和利什曼原虫菌株具有显著的生物学效力。此外，还阐明了 Ag-NPs 的相当大的抗糖尿病、抗癌、抗氧化和生物相容性特性。Ag-NPs 具有最高的抗氧化活性，在 200μg/ml 时，TAC、TRP DPPH 的抗氧化活性分别为 50.61±1.12%、38.22±1.18%和 27.39±0.92%。Ag-NPs 对前鞭毛体的杀利什曼原虫活性为 80%±1.4%，对无鞭毛体的杀利什曼原虫活性为 77%±1.6%。在 200μg/ml 时，抗糖尿病活性最高，为 30±0.77%。Ag-NPs 对盐水虾的最大细胞毒性为 200μg/ml 时的 60±1.18%。Ag-NPs 的最大染料降解率在 140 分钟时记录为 94.1%。所有尿路感染分离株均对未涂有 Ag-NPs 的抗生素耐药。Ag-NPs 的最高活性为 1%时，对 的抑制率为 25±1.58mm。Ag-NPs 涂有亚胺培南抗生素的最大抑菌环为 26±1.5mm，涂有环丙沙星的抑菌环为 26±1.4mm。最后但并非最不重要的是，Ag-NPs 对新鲜 RBC 表现出高度的生物相容性，使生态友好型生物合成的银 NPs 成为生物医学领域的多维度候选物。
Zotero 插件