Cardona-Sanclemente L E, Verneau C, Mathe D, Lutton C
Biochim Biophys Acta. 1987 Jun 23;919(3):205-12. doi: 10.1016/0005-2760(87)90259-1.
The rates of mobile cholesterol turnover processes were measured by the isotopic equilibrium method in normocholesterolemic (SW) and hypercholesterolemic homozygote (RICO) rats fed a semi-synthetic base diet containing 0.05% cholesterol. When the absorption rate is similar in SW and RICO rats, the internal secretion rate is 60% higher in RICO (25.3 mg/day) than in SW (16.2 mg/day). This increase is compensated by an increase in fecal excretion (RICO: 5 mg/day; SW: 3.8 mg/day), urinary excretion (RICO: 1.7 mg/day; SW: 1.1 mg/day) and above all the transformation of cholesterol into bile acids (RICO: 24.2 mg/day; SW: 15.3 mg/day). The fact that 70 minutes after [14C]acetate administration, the only variations obtained in RICO compared to SW rats are a doubled sterol radioactivity in the small intestine and a tripled one in the liver suggests that the increase in internal secretion of the RICO rat has both an intestinal and hepatic origin. This cholesterogenic stimulation in RICO rats takes place in the jejunum as well as in the ileum and in the crypt cells as well as in the villosities. It is concomitant with a doubled cholesterolemia, a doubled intestinal, caecal and colon bile acid pool and a 20% increase in the enterocyte protein content.
采用同位素平衡法，对喂食含0.05%胆固醇的半合成基础日粮的正常胆固醇血症（SW）大鼠和高胆固醇血症纯合子（RICO）大鼠的流动胆固醇周转过程速率进行了测定。当SW和RICO大鼠的吸收率相似时，RICO大鼠的内源性分泌率（25.3毫克/天）比SW大鼠（16.2毫克/天）高60%。这种增加通过粪便排泄增加（RICO：5毫克/天；SW：3.8毫克/天）、尿液排泄增加（RICO：1.7毫克/天；SW：1.1毫克/天），尤其是胆固醇向胆汁酸的转化增加（RICO：24.2毫克/天；SW：15.3毫克/天）来补偿。在给予[14C]乙酸盐70分钟后，与SW大鼠相比，RICO大鼠唯一的变化是小肠中固醇放射性增加一倍，肝脏中增加两倍，这一事实表明，RICO大鼠内源性分泌的增加既有肠道来源也有肝脏来源。RICO大鼠的这种胆固醇生成刺激发生在空肠以及回肠，发生在隐窝细胞以及绒毛中。它伴随着胆固醇血症增加一倍、肠道、盲肠和结肠胆汁酸池增加一倍以及肠上皮细胞蛋白质含量增加20%。
