Dazord L, Bourel D, Martin A, Lecorre R, Bourguet P, Bohy J, Saccavini J C, Delaval P, Louvet M, Toujas L
Cancer Immunol Immunother. 1987;24(3):263-8. doi: 10.1007/BF00205641.
Po66, a mouse IgG1 monoclonal antibody, was produced by immunization against a patient lung squamous cell carcinoma. The tissue reactivity of the antibody was measured by a radioimmunological assay with enzymatically dissociated cells, by an immunofluorescence test on frozen tissue sections and by peroxidase-staining of paraffin sections. The antibody bound to lung squamous cell carcinoma, oesophagus carcinoma and, inconsistently to lung adenocarcinoma but not to the other tumours tested. Some normal tissues also reacted positively, in particular bronchial serous glands, oesophagus epithelium and renal distal and collecting tubules. In normal and malignant tissues showing epithelioid differentiation, Po66 bound to the intermediate maturation area. The antigen immunoprecipitated by Po66 from lung squamous cell carcinoma appeared as a single band with a molecular weight 47,000 to 50,000 daltons. Purified monoclonal antibody Po66 and an unrelated IgG1 immunoglobulin were labelled with radioactive iodine and injected i.v. into nude mice bearing subcutaneous xenografts of human lung squamous cell carcinoma. The localization index in the tumour was 3.3. Antibody labelled with 131I allowed gamma-scintigraphic imaging of the xenografts which were clearly outlined by days 9 to 11.
Po66是一种小鼠IgG1单克隆抗体，通过用一名肺鳞状细胞癌患者的肿瘤进行免疫接种制备而成。该抗体的组织反应性通过以下方法测定：用酶解离细胞进行放射免疫分析、对冷冻组织切片进行免疫荧光检测以及对石蜡切片进行过氧化物酶染色。该抗体与肺鳞状细胞癌、食管癌结合，偶尔也与肺腺癌结合，但不与其他所检测的肿瘤结合。一些正常组织也呈阳性反应，特别是支气管浆液腺、食管上皮以及肾远曲小管和集合小管。在显示上皮样分化的正常和恶性组织中，Po66与中间成熟区域结合。Po66从肺鳞状细胞癌中免疫沉淀出的抗原表现为一条分子量为47,000至50,000道尔顿的单带。将纯化的单克隆抗体Po66和一种无关的IgG1免疫球蛋白用放射性碘标记，静脉注射到携带人肺鳞状细胞癌皮下异种移植物的裸鼠体内。肿瘤中的定位指数为3.3。用131I标记的抗体可对异种移植物进行γ闪烁成像，到第9至11天时可清晰勾勒出其轮廓。
