来自恶性疾病患者的人杂交瘤。
Human hybridomas from patients with malignant disease.
作者信息
Sikora K, Alderson T, Ellis J, Phillips J, Watson J
出版信息
Br J Cancer. 1983 Jan;47(1):135-45. doi: 10.1038/bjc.1983.16.
Abstract
Lymphocytes from 180 patients with a variety of malignant diseases were collected and fused with a human myeloma-derived line, LON-LICR-HMy2/CAM1. A total of 162 hybridomas was obtained. Only B lymphocyte markers were found on the surface of the fusion products. Flow cytometric analysis revealed a stably increased DNA content in the hybridoma cells. Some hybridoma supernatants were found to contain new Ig chains. Anti-tumour binding activity was found in 12 supernatants.
摘要
收集了180例患有各种恶性疾病患者的淋巴细胞，并将其与源自人骨髓瘤的LON-LICR-HMy2/CAM1细胞系进行融合。共获得162个杂交瘤。在融合产物表面仅发现B淋巴细胞标志物。流式细胞术分析显示杂交瘤细胞中的DNA含量稳定增加。发现一些杂交瘤培养上清液含有新的免疫球蛋白链。在12种培养上清液中发现了抗肿瘤结合活性。
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