Gu Jia, Chen Yu-Zhong, Zhang Zi-Xiang, Yang Zai-Xing, Duan Guang-Xin, Qin Li-Qiang, Zhao Lin, Xu Jia-Ying
State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, Jiangsu, China.
State Key Laboratory of Radiation Medicine and Protection, Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China.
Dose Response. 2020 Sep 10;18(3):1559325820956783. doi: 10.1177/1559325820956783. eCollection 2020 Jul-Sep.
To investigate the doses of total body (TBI) and whole abdominal irradiation (WAI) induced lethal intestinal injury, healthy C57BL/6 J mice were divided randomly into 7 groups: control group; 6, 7, and 8 Gy TBI groups; and 5, 10, and 15 Gy WAI groups. The survival length, general conditions, body weight, daily food and water intake of the mice and the histopathological changes of small intestine were observed.
Lethal injury among C57BL/6 J mice was caused by ≥6 Gy TBI and 15 Gy WAI. Their body weight and food intake decreased, the structure of their small intestinal villi was destroyed, and the number of surviving crypts per circumference of the jejunum decreased in ≥6 Gy TBI groups and 15 Gy WAI group. The mice in the 10 Gy WAI group significantly lost weight within 5 days but recovered slowly thereafter. They also had poor appetite and reversibly damaged intestinal mucosa.
Nonlethal intestinal injury could be induced by 10 Gy WAI, whereas lethal intestinal injury could be triggered by ≥6 Gy TBI and >15 Gy WAI in mice. Our results provided a basis for establishing radiation-induced intestinal injury models with C57BL/6 J mice.
为研究全身照射（TBI）和全腹照射（WAI）诱导致死性肠损伤的剂量，将健康的C57BL/6 J小鼠随机分为7组：对照组；6、7和8 Gy TBI组；以及5、10和15 Gy WAI组。观察小鼠的存活时长、一般状况、体重、每日食物和水摄入量以及小肠的组织病理学变化。
≥6 Gy TBI和15 Gy WAI可导致C57BL/6 J小鼠发生致死性损伤。≥6 Gy TBI组和15 Gy WAI组小鼠体重和食物摄入量下降，小肠绒毛结构被破坏，空肠每周长存活隐窝数量减少。10 Gy WAI组小鼠在5天内体重显著下降，但此后恢复缓慢。它们还食欲不佳，肠黏膜有可逆性损伤。
10 Gy WAI可诱导小鼠发生非致死性肠损伤，而≥6 Gy TBI和>15 Gy WAI可引发小鼠致死性肠损伤。我们的结果为建立C57BL/6 J小鼠辐射诱导肠损伤模型提供了依据。
