Bellec Laure, Cambon-Bonavita Marie-Anne, Durand Lucile, Aube Johanne, Gayet Nicolas, Sandulli Roberto, Brandily Christophe, Zeppilli Daniela
Ifremer, Centre Brest, REM/EEP/LEP, ZI de la Pointe du Diable, CS10070, Plouzané, France.
Laboratoire de Microbiologie des Environnements Extrêmes, Ifremer, CNRS, Univ Brest, Plouzané, France.
Front Microbiol. 2020 Aug 20;11:2023. doi: 10.3389/fmicb.2020.02023. eCollection 2020.
Shallow-water hydrothermal vents are widespread, especially in the Mediterranean Sea, owing to the active volcanism of the area. Apart free microbial communities' investigations, few biological studies have been leaded yet. Investigations of microbial communities associated with Nematoda, an ecologically important group in sediments, can help to improve our overall understanding of these ecosystems. We used a multidisciplinary-approach, based on microscopic observations (scanning electron microscopy: SEM and Fluorescence Hybridization: FISH) coupled with a molecular diversity analysis using metabarcoding, based on the 16S rRNA gene (V3-V4 region), to characterize the bacterial community of a free-living marine nematode and its environment, the shallow hydrothermal vent near Naples (Italy). Observations of living bacteria in the intestine (FISH), molecular and phylogenetic analyses showed that this species of nematode harbors its own bacterial community, distinct from the surrounding sediment and water. Metabarcoding results revealed the specific microbiomes of the sediment from three sites of this hydrothermal area to be composed mainly of sulfur oxidizing and reducing related bacteria.
由于该地区活跃的火山活动，浅水热液喷口分布广泛，尤其是在地中海。除了对微生物群落的研究外，目前尚未开展其他生物学研究。对线虫（沉积物中一个具有重要生态意义的类群）相关微生物群落的研究，有助于提高我们对这些生态系统的整体认识。我们采用了多学科方法，基于显微镜观察（扫描电子显微镜：SEM和荧光原位杂交：FISH），并结合基于16S rRNA基因（V3 - V4区域）的元条形码分子多样性分析，来表征一种自由生活的海洋线虫及其环境（意大利那不勒斯附近的浅水热液喷口）的细菌群落。对肠道内活细菌的观察（FISH）、分子和系统发育分析表明，这种线虫拥有自己独特的细菌群落，与周围沉积物和水体中的细菌群落不同。元条形码分析结果显示，该热液区三个地点沉积物的特定微生物群主要由与硫氧化和还原相关的细菌组成。
