Shanthini Thamaraiselvan, Manohar Prasanth, Samna Sagadevan, Srividya Rajendran, Bozdogan Bulent, Rameshpathy Manian, Ramesh Nachimuthu
Antibiotic Resistance and Phage Therapy Laboratory, Department of Biomedical Sciences, School of Bioscience and Technology, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu, India.
Department of Microbiology, Recombinant DNA and Protein Research Center (REDPROM), Adnan Menderes University, 09100 Aydın, Turkey.
Access Microbiol. 2019 May 15;1(3):e000024. doi: 10.1099/acmi.0.000024. eCollection 2019.
Wastewater has become a potential habitat for multi-drug-resistant bacteria. The present study aims to screen for the presence of carbapenem-resistant bacteria in sewage water samples collected from hospital and non-hospital sources. From a total of 19 sewage water samples collected, 100 carbapenem-resistant non-lactose-fermenting Gram-negative bacteria (CR-NF-GNB) were isolated using MacConkey agar cultured with 8 mg l of meropenem. On screening for beta-lactamase resistance genes ( , , , and ), one isolate, , was found to carry the plasmid-borne gene. To the best of our knowledge, we provide the first report of the rare and emerging opportunistic pathogen encoding the OXA-181 gene in its plasmid.
废水已成为耐多药细菌的潜在栖息地。本研究旨在筛查从医院和非医院来源采集的污水样本中耐碳青霉烯类细菌的存在情况。在总共采集的19份污水样本中,使用添加了8毫克/升美罗培南的麦康凯琼脂分离出了100株耐碳青霉烯类非乳糖发酵革兰氏阴性菌(CR-NF-GNB)。在筛查β-内酰胺酶耐药基因(blaOXA、blaVIM、blaIMP、blaNDM和blaKPC)时,发现一株名为Sphingobacterium multivorum的分离株携带质粒介导的blaOXA-181基因。据我们所知,我们首次报道了这种罕见且新出现的机会性病原体Sphingobacterium multivorum在其质粒中编码OXA-181基因。