布雷菲德菌素A和细胞松弛素B可减少登革病毒在细胞培养物中的复制，但不能保护小鼠免受病毒攻击。
Brefeldin A and Cytochalasin B reduce dengue virus replication in cell cultures but do not protect mice against viral challenge.
作者信息
Farias Kleber Juvenal Silva, Machado Paula Renata Lima, de Almeida Júnior Renato Ferreira, Lopes da Fonseca Benedito Antônio
机构信息
Department of Internal Medicine, School of Medicine of Ribeirao Preto - University of Sao Paulo, Avenida Bandeirantes, 3900, Monte Alegre, 14049-900, Ribeirao Preto SP, Brazil.
Program of Graduate Studies on Applied Microbiology and Immunology, School of Medicine of Ribeirao Preto - University of Sao Paulo, Avenida Bandeirantes, 3900, Monte Alegre, 14049-900, Ribeirão Preto SP, Brazil.
出版信息
Access Microbiol. 2019 Jul 22;1(6):e000041. doi: 10.1099/acmi.0.000041. eCollection 2019.
BACKGROUND
Dengue is an emerging arboviral disease caused by dengue virus (DENV). DENV belongs to the family and genus . No specific anti-DENV drugs are currently available.
METHODS
We investigated the antiviral activity of Brefeldin A (BFA) and Cytochalasin B (CB) against this infection. The drugs BFA and CB were used in the treatment of dengue-2 virus (DENV-2) infections in Vero cell cultures and in protection from lethality by post-challenge administration in Swiss mice. Viral load was quantified by qRT-PCR and plaque assay in Vero cell cultures, post-infection, treated or not with the drugs. Post-challenge drug levels were evaluated by survival analysis.
RESULTS
Our results indicate that doses of 5 µg ml of BFA and 10 µg ml of CB are not toxic to the cells and induce a statistically significant inhibition of DENV-2 replication in Vero cells when compared to control. No BFA- or CB-treated mice survived the challenge with DENV-2.
CONCLUSION
These data suggest that BFA and CB have an antiviral action against DENV-2 replication in Vero cell culture, but do not alter infected mice mortality.
背景
登革热是由登革病毒（DENV）引起的一种新发虫媒病毒病。DENV属于 科 属。目前尚无特异性抗DENV药物。
方法
我们研究了布雷菲德菌素A（BFA）和细胞松弛素B（CB）对这种感染的抗病毒活性。BFA和CB药物用于在Vero细胞培养物中治疗登革热2型病毒（DENV-2）感染，并通过在瑞士小鼠中攻毒后给药来保护其免受致死性感染。在病毒感染后，用或不用药物处理的Vero细胞培养物中，通过qRT-PCR和噬斑测定法定量病毒载量。通过生存分析评估攻毒后药物水平。
结果
我们的结果表明，与对照相比，5 μg/ml的BFA和10 μg/ml的CB剂量对细胞无毒，并在Vero细胞中诱导对DENV-2复制有统计学意义的抑制作用。用BFA或CB处理的小鼠在DENV-2攻毒后均未存活。
结论
这些数据表明，BFA和CB对Vero细胞培养物中的DENV-2复制具有抗病毒作用，但不会改变感染小鼠的死亡率。
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