Student Research Committee, Iran University of Medical Sciences, Tehran, Iran.
Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Nanomedicine (Lond). 2020 Aug;15(23):2255-2270. doi: 10.2217/nnm-2020-0119. Epub 2020 Sep 25.
In this study, the effects of ionizing radiation and 5-fluorouracil (5-FU)-loaded triblock copolymer-coated magnetic nanoparticles (NPs) on the induction of apoptosis in HT-29 and HCT-116 were investigated. The percentage of apoptotic cells and alteration of the expression of apoptotic-related proteins were evaluated in treated cells by flow cytometry and western blot analysis, respectively. Combination treatment with 5-FU and radiation had a stronger effect on decreasing Bcl-2 expression and increasing expression of Bax, cleaved caspase-9, cleaved caspase-3, cleaved PARP compared with each treatment alone. The combination of radiation and triblock copolymer-coated magnetic NPs as 5-FU drug carriers works by triggering apoptosis to improve treatment efficacy. Additional study may present the NPs as an effective approach for the treatment of colon cancer.
在这项研究中，研究了电离辐射和 5-氟尿嘧啶（5-FU）负载的三嵌段共聚物包覆磁性纳米粒子（NPs）对 HT-29 和 HCT-116 细胞凋亡诱导的影响。 通过流式细胞术和 Western blot 分析分别评估了处理细胞中凋亡细胞的百分比和凋亡相关蛋白表达的变化。 与单独每种治疗相比，5-FU 和辐射的联合治疗对降低 Bcl-2 表达和增加 Bax、裂解 caspase-9、裂解 caspase-3、裂解 PARP 的表达有更强的作用。 辐射和三嵌段共聚物包覆磁性 NPs 作为 5-FU 药物载体的组合通过触发细胞凋亡来提高治疗效果。 进一步的研究可能会将 NPs 作为治疗结肠癌的有效方法。
