Lu C H, Chang S N, Tsai M Y
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1987 Feb;20(1):37-45.
Mucin has been used as virulence-enhancing (VE) agent in estimation of the toxicity of bacteria to mice and the potency of bacterial vaccines such as typhoid vaccine. Discontinuance of the acceptable brand of mucin made it necessary to search for an effective alternative. Five percent inactivated Saccharomyces cerevisiae (IY) showed a significant higher VE effect on S. typhi in mice (132 colony forming units/LD50) with lower viscosity when compared to 5% mucin (740 colony forming units/LD50), and either one could promote bacterial lethal toxicity in mice more than 10(4) times. The strong correlation (r2 = 0.9765) between the immune dose and the ratio of mouse death was found by using 5% IY as VE agent in the active mouse protection test for typhoid vaccine. The relative potencies of a typhoid vaccine to the reference vaccine obtained by using 5% mucin or 5% IY as VE agent were not significant difference. Therefore, IY was a suitable substitute for mucin as a VE agent in the toxicity test of bacteria and in the immunity assay of typhoid vaccines.
黏蛋白已被用作毒力增强（VE）剂，用于评估细菌对小鼠的毒性以及诸如伤寒疫苗等细菌疫苗的效力。可接受品牌的黏蛋白停产，使得寻找有效的替代品成为必要。与5%黏蛋白（740个菌落形成单位/LD50）相比，5%灭活酿酒酵母（IY）对小鼠体内伤寒杆菌的VE效应显著更高（132个菌落形成单位/LD50），且黏度更低，二者均可使小鼠体内细菌致死毒性提高超过10^4倍。在伤寒疫苗的主动小鼠保护试验中，使用5%IY作为VE剂时，发现免疫剂量与小鼠死亡比例之间存在强相关性（r2 = 0.9765）。使用5%黏蛋白或5%IY作为VE剂获得的伤寒疫苗相对于参考疫苗的相对效力无显著差异。因此，在细菌毒性试验和伤寒疫苗免疫测定中，IY是黏蛋白作为VE剂的合适替代品。
