Maizels R M, Selkirk M E, Sutanto I, Partono F
Ciba Found Symp. 1987;127:189-202. doi: 10.1002/9780470513446.ch13.
The lymphatic filarial parasites, Brugia and Wuchereria, continue to present an immunological puzzle, particularly with respect to the development of natural resistance or damaging disease. We have approached this question by examining humoral responses to a few defined antigens of selected interest from these parasites, using sera from each category in the spectrum of filarial disease. Many antigens, such as the major adult surface protein of Mr 29,000 (29K), appear to be recognized at all stages of infection, but two components show interesting patterns of differential recognition. A triplet of proteins of Mr 65-75K associated with the microfilarial surface is preferentially bound by serum from patent microfilaraemic infections, whereas an unrelated 75K protein has been found to react only with antibody from amicrofilaraemic individuals. In general, however, the data obtained so far emphasize the importance of undertaking an antigenic analysis at the level of single epitopes. Such studies are now under way using recombinant proteins expressed in bacterial hosts.
淋巴丝虫寄生虫，如布鲁氏丝虫和班氏丝虫，仍然是一个免疫学难题，特别是在自然抵抗力的发展或破坏性疾病方面。我们通过检测对这些寄生虫中选定的几种特定抗原的体液反应来探讨这个问题，使用来自丝虫病谱系中每个类别的血清。许多抗原，如分子量为29,000（29K）的主要成虫表面蛋白，似乎在感染的所有阶段都能被识别，但有两种成分表现出有趣的差异识别模式。与微丝蚴表面相关的一组分子量为65 - 75K的蛋白质优先被显性微丝蚴血症感染的血清所结合，而一种不相关的75K蛋白质已被发现仅与无微丝蚴血症个体的抗体发生反应。然而，总体而言，目前获得的数据强调了在单个表位水平上进行抗原分析的重要性。目前正在使用在细菌宿主中表达的重组蛋白进行此类研究。
