Maizels R, Burke J, Sutanto I, Partono F
Mol Biochem Parasitol. 1986 Apr;19(1):27-34. doi: 10.1016/0166-6851(86)90062-9.
Antigenic proteins of microfilariae and infective larvae of Wuchereria bancrofti have been identified by intrinsic and extrinsic radiolabelling, and specific immunoprecipitation with sera from filarial patients. From 125I surface-labelling experiments, the most prominent antigen on both stages is of relative molecular mass (Mr) 17 000, while a molecule of similar size is both synthesized and released in vitro following labelling with [35S]methionine. A second similarity between the two stages is the production and secretion of a Mr 21 000 component, which is, however, not detected on the worm surfaces. A series of additional proteins from larval W. bancrofti are described from each parasite compartment (secreted, surface and somatic) and the antigenicity and specificity of these components explored with serum from patients with filariasis due to W. bancrofti or Brugia species, and with onchocerciasis. Among additional molecules released in vitro we have found a Mr 51 000 antigen from both stages, and also several proteins which are not recognised by antibody from human filarial patients.
通过内源性和外源性放射性标记以及用丝虫病患者血清进行特异性免疫沉淀，已鉴定出班氏吴策线虫微丝蚴和感染性幼虫的抗原蛋白。从¹²⁵I表面标记实验可知，两个阶段最突出的抗原相对分子质量（Mr）为17000，而在用[³⁵S]甲硫氨酸标记后，体外合成并释放出一种大小相似的分子。这两个阶段的第二个相似之处是产生并分泌一种Mr为21000的成分，不过在虫体表面未检测到。从班氏吴策线虫幼虫的每个寄生部位（分泌的、表面的和体细胞的）描述了一系列额外的蛋白质，并利用班氏吴策线虫或布鲁氏丝虫引起的丝虫病患者以及盘尾丝虫病患者的血清，探究了这些成分的抗原性和特异性。在体外释放的其他分子中，我们发现两个阶段都有一种Mr为51000的抗原，还有几种蛋白质不被人类丝虫病患者的抗体识别。
