Laboratoire de Microbiologie Appliquée, Faculté des Sciences la Nature et de la Vie, Université de Bejaia, 06000, Bejaia, Algeria.
BioEcoAgro N° 1158, Univ. Lille, INRAE, Univ. Liège, UPJV, YNCREA, Univ. Artois, Univ. Littoral Côte d'Opale, ICV, Institut Charles Viollette, F-59000, Lille, France.
Microb Ecol. 2021 Nov;82(4):1020-1029. doi: 10.1007/s00248-020-01606-6. Epub 2020 Sep 25.
The composition of the vaginal microbiota is a key element for maintaining gynecological and reproductive health. With the aim of obtaining an accurate overview of the vaginal microbiota of Algerian women, in terms of their age and ethnic group, we conducted a 16S rRNA gene targeted metagenomic analysis of 100 vaginal samples taken from healthy childbearing and menopausal women. These data were used to establish the pattern of the vaginal microbiota during reproductive and postreproductive phases. Hormone levels were correlated to changes in microbial composition for menopausal women. The ethnic comparison revealed a particular microbiota profile for Algerian women, with a dominance of CST III and CST I. A rapid qPCR method developed by the authors was successfully used to identify the vaginal bacterial pattern for a customized gynecological management.
阴道微生物组的组成是维持妇科和生殖健康的关键因素。为了全面了解阿尔及利亚女性的阴道微生物组，我们针对年龄和种族进行了 16S rRNA 基因靶向宏基因组分析，对 100 例来自健康育龄期和绝经期妇女的阴道样本进行了分析。这些数据用于建立生殖和生殖后阶段阴道微生物组的模式。对绝经期妇女，激素水平与微生物组成的变化相关。种族比较显示，阿尔及利亚妇女具有独特的阴道微生物组特征，以 CST III 和 CST I 为主导。作者开发的快速 qPCR 方法成功地用于识别阴道细菌模式，用于定制的妇科管理。
