MOR-1 细胞分布的荧光共定位分析。
Fluorescence Colocalization Analysis of Cellular Distribution of MOR-1.
机构信息
Department of Neuroscience, Psychology, Drug Research and Child Health (Neurofarba), University of Florence, Florence, Italy.
出版信息
Methods Mol Biol. 2021;2201:27-34. doi: 10.1007/978-1-0716-0884-5_3.
PMID:32975786
Abstract
The interaction between neurons and glia is pivotal for the development of chronic opioid tolerance. One of the most important mechanisms of cell-to-cell interaction is the Notch signaling pathway. In this chapter we propose a double-immunofluorescence method to observe and quantify the colocalization of Notch-1 and mu-opioid receptor (MOR-1), using both neuronal and astrocyte markers.
摘要
神经元和神经胶质细胞之间的相互作用对于慢性阿片类药物耐受的发展至关重要。细胞间相互作用的最重要机制之一是 Notch 信号通路。在本章中，我们提出了一种双重免疫荧光方法，使用神经元和星形胶质细胞标志物来观察和量化 Notch-1 和 μ-阿片受体 (MOR-1) 的共定位。
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