Cheng M, Yin H Z, Feng X Q
Zhonghua Zhong Liu Za Zhi. 1987 Jan;9(1):14-6.
CEA McAbs, recognizing three different epitopes on CEA molecules, were used to measure serum CEA level in cancer patients by enzyme-immunoassay (EIA). The results, as compared with those using polyclonal antibodies, indicated that the positive rate was higher while the false positivity was lower. Immunohistochemistry of tumour sections showed that the CEA McAbs are bonded to 80-90% of gastrointestinal cancers. Although the normal colon epithelium occasionally reacted with CEA McAbs, other normal tissues did not. After in vivo administration of radio-labeled CEA McAbs to nude mice xenograft with human colon cancer, the radio-isotope was found to be concentrated preferentially in the tumour. The ratio of tumour and normal tissue was 3.6-11.8 after 48 hours following administration. Thus, the CEA McAbs can be used clinically not only for serum CEA determination but also for diagnostic imaging.
识别癌胚抗原（CEA）分子上三种不同表位的CEA单克隆抗体，通过酶免疫测定法（EIA）用于检测癌症患者血清中的CEA水平。与使用多克隆抗体的结果相比，结果表明阳性率更高而假阳性率更低。肿瘤切片的免疫组织化学显示，CEA单克隆抗体与80%-90%的胃肠道癌结合。虽然正常结肠上皮偶尔会与CEA单克隆抗体发生反应，但其他正常组织则不会。将放射性标记的CEA单克隆抗体体内给药至接种人结肠癌的裸鼠后，发现放射性同位素优先集中在肿瘤中。给药后48小时，肿瘤与正常组织的比例为3.6-11.8。因此，CEA单克隆抗体不仅可在临床上用于血清CEA的测定，还可用于诊断成像。
