Skinner M K, Keski-Oja J, Osteen K G, Moses H L
Endocrinology. 1987 Aug;121(2):786-92. doi: 10.1210/endo-121-2-786.
Ovarian thecal cells in culture were found to synthesize and secrete transforming growth factor-beta (TGF beta). A component in thecal cell-conditioned medium was immunologically similar to TGF beta, as assessed with a RIA, and inhibited specific binding of TGF beta to its cell surface receptors. Thecal cell-secreted proteins also contained TGF beta biological activity, which was determined by stimulation of soft agar colony formation by AKR-2B indicator cells. Specific TGF beta antibodies precipitated a 25 K protein from radiolabeled thecal cell-secreted protein that comigrated with purified platelet-derived TGF beta. Both bovine thecal cell and rat thecal/interstitial cell preparations produced TGF beta, which required acid treatment to obtain fully active samples. The physiological significance of TGF beta production by thecal cells was addressed through an analysis of the effects of TGF beta on bovine granulosa cell growth. TGF beta inhibited epidermal growth factor stimulation of granulosa cell growth, but alone it had no apparent influence. Observations indicate that ovarian thecal cells produce TGF beta, which can regulate granulosa cell growth and differentiation. Discussion of thecal cell-granulosa cell interactions and the possible functions of TGF beta in the ovary is presented.
研究发现，培养的卵巢膜细胞能够合成并分泌转化生长因子-β（TGF-β）。用放射免疫分析法评估发现，膜细胞条件培养基中的一种成分在免疫学上与TGF-β相似，并且能够抑制TGF-β与其细胞表面受体的特异性结合。膜细胞分泌的蛋白质还具有TGF-β生物活性，这是通过AKR-2B指示细胞刺激软琼脂集落形成来确定的。特异性TGF-β抗体从放射性标记的膜细胞分泌蛋白中沉淀出一种25K的蛋白质，该蛋白质与纯化的血小板衍生TGF-β迁移率相同。牛膜细胞和大鼠膜/间质细胞制剂均产生TGF-β，需要进行酸处理才能获得完全活性的样品。通过分析TGF-β对牛颗粒细胞生长的影响，探讨了膜细胞产生TGF-β的生理意义。TGF-β抑制表皮生长因子对颗粒细胞生长的刺激作用，但单独使用时没有明显影响。观察结果表明，卵巢膜细胞产生TGF-β，其可以调节颗粒细胞的生长和分化。本文讨论了膜细胞-颗粒细胞相互作用以及TGF-β在卵巢中的可能功能。
