Department of Second Obstetrics, Cangzhou Central Hospital, No. 16 Xinhua West Road, Cangzhou 061000, Hebei, China.
Department of Brain Emergency, Cangzhou Central Hospital, No. 16 Xinhua West Road, Cangzhou 061000, Hebei, China.
Prostaglandins Leukot Essent Fatty Acids. 2020 Oct;161:102179. doi: 10.1016/j.plefa.2020.102179. Epub 2020 Sep 19.
Accumulatig evidence demonstrated that inflammation is associated with the development of gestational diabetes mellitus (GDM). Fatty acid-binding protein 4 (FABP4) was reported to be involved in immune response. However, the effect of FABP4 in GDM remians unclear. This study focused on the effect of FABP4 in GDM. C57BL/KsJdb/+ (db/+) mice were used for GDM mouse model . BMS-309403 (BMS) was used to inhibit FABP4 levels in GDM mouse model. IL-6 and TNF-α concentrations in serum were determined via ELISA. Serum glucose and insulin concentrations were tested using commercial glucometer and mouse insulin ELISA kit, respectively. IL-6 and TNF-α mRNA and protein levels were detected using RT-PCR and western blot, respectively. FABP4 levels were upregulated in GDM group compared with control group and were positively associated with serum IL-6 and TNF-α levels. FABP4 inhibition by BMS significantly decreased body weight and serum glucose concentrations, increasd serum insulin concentration, suppressed IL-6 and TNF-α expression both in the serum and the pancreas, enhanced little size and inhibited birth weight in GDM mouse model. Inhibition of FABP4 attenuates GDM in genetic mice.
越来越多的证据表明炎症与妊娠期糖尿病（GDM）的发生有关。脂肪酸结合蛋白 4（FABP4）被报道参与免疫反应。然而，FABP4 在 GDM 中的作用尚不清楚。本研究旨在探讨 FABP4 在 GDM 中的作用。使用 C57BL/KsJdb/+（db/db+）小鼠建立 GDM 小鼠模型。用 BMS-309403（BMS）抑制 GDM 小鼠模型中的 FABP4 水平。通过 ELISA 法测定血清中 IL-6 和 TNF-α的浓度。使用商业血糖仪和小鼠胰岛素 ELISA 试剂盒分别检测血清葡萄糖和胰岛素浓度。采用 RT-PCR 和 Western blot 法分别检测 IL-6 和 TNF-α的 mRNA 和蛋白水平。与对照组相比，GDM 组 FABP4 水平升高，且与血清 IL-6 和 TNF-α水平呈正相关。BMS 抑制 FABP4 可显著降低体重和血清葡萄糖浓度，增加血清胰岛素浓度，抑制 GDM 小鼠模型血清和胰腺中 IL-6 和 TNF-α的表达，减小胰岛体积并抑制出生体重。FABP4 的抑制可减轻遗传型 GDM 小鼠的病情。
