Burrin J M, Uttenthal L O, McGregor G P, Bloom S R
Experientia. 1987 Jul 15;43(7):734-41. doi: 10.1007/BF01945350.
Although almost all methods of mass measurement of regulatory peptides still depend on the high affinity antibody, the traditional Yalow and Berson radioimmunoassay technique is becoming outdated. Pure monoclonal antibodies allow excess antibody two site assay techniques with a variety of different labels (preferentially non-radioactive) of great sensitivity and speed. The large amounts of particular monoclonal antibodies available allow several different laboratories to use the same reagents and have increased comparability. Unfortunately many regulatory peptides exist in multiple molecular forms and attention must be paid to antibody region specificity. Improved methods of extraction of regulatory peptides from plasma tissue allow more accurate quantitation. New techniques for rapid high resolution chromatography make distinction of different molecular forms much easier than hitherto. Better education in techniques and/or attention to inter-assay standards are necessary to improve the comparability of regulatory peptide measurement in the future.
尽管几乎所有调节肽的大规模测量方法仍依赖于高亲和力抗体，但传统的雅洛和伯森放射免疫分析技术已逐渐过时。纯单克隆抗体使得采用具有多种不同标记（优先选择非放射性标记）的过量抗体双位点分析技术成为可能，这些技术灵敏度高且速度快。大量可得的特定单克隆抗体使多个不同实验室能够使用相同试剂，从而提高了可比性。不幸的是，许多调节肽以多种分子形式存在，必须注意抗体区域特异性。从血浆组织中提取调节肽的改进方法可实现更准确的定量。快速高分辨率色谱新技术使区分不同分子形式比以往任何时候都更容易。为提高未来调节肽测量的可比性，有必要加强技术培训和/或关注分析间标准。
