Enzyme-immunoassay: a review.

The main principles of heterogeneous and homogeneous enzyme-immunoassays are reviewed. Furthermore, an overview is given of enzymes currently used as labels and methods applied for conjugate preparation. The reliability and practicability of enzyme-immunoassays are discussed. Special consideration is given to possible interferences, the detection limits and the mechanization of these assays. A range of curve-fitting methods for evaluation of the results is listed. Future prospects for the further development of enzyme-immunoassay and application of this technique are discussed.