State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China.
State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China.
Vet Microbiol. 2020 Oct;249:108848. doi: 10.1016/j.vetmic.2020.108848. Epub 2020 Sep 17.
Porcine epidemic diarrhea (PED) is a devastating enteric disease to the world's swine production. Porcine epidemic diarrhea virus (PEDV), as the PED causative agent, has been commonly propagated and investigated in Vero cells, as well as in IPEC-J2, a porcine epithelial cell-jejunum 2. However, Vero cells, which are defective in interferon production, cannot represent the host response in enteric cells while PEDV replicates poorly in IPEC-J2 cells. In this study, we observed that rat crypt epithelial cells (IEC-6) were highly susceptible to different subtypes of PEDV. The replication kinetics of PEDV in IEC-6 cells is similar to that in Vero cells, but it is much higher than in IPEC-J2 cells. Besides that, PEDV infection in IEC-6 cells can induce the production of inflammatory cytokines and interferon, especially the type III IFNs. Collectively, our findings suggest that IEC-6 is an ideal cell line for PEDV replication and immune response studies.
猪流行性腹泻（PED）是一种严重的肠道疾病，对全球的养猪业造成了巨大的影响。猪流行性腹泻病毒（PEDV）是 PED 的病原体，已在 Vero 细胞以及猪肠上皮细胞-空肠 2 号（IPEC-J2）中得到广泛的繁殖和研究。然而，Vero 细胞缺乏干扰素的产生，无法代表肠道细胞中的宿主反应，同时 PEDV 在 IPEC-J2 细胞中复制能力较差。在本研究中，我们观察到大鼠隐窝上皮细胞（IEC-6）对不同亚型的 PEDV 高度易感。PEDV 在 IEC-6 细胞中的复制动力学与在 Vero 细胞中的相似，但比在 IPEC-J2 细胞中的高得多。此外，PEDV 感染 IEC-6 细胞可以诱导炎症细胞因子和干扰素的产生，特别是 III 型干扰素。综上所述，我们的研究结果表明 IEC-6 是研究 PEDV 复制和免疫反应的理想细胞系。
