College of Agriculture, Guizhou University, Guiyang 550025, PR China.
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, PR China; College of Agriculture, Guizhou University, Guiyang 550025, PR China.
Pestic Biochem Physiol. 2020 Nov;170:104681. doi: 10.1016/j.pestbp.2020.104681. Epub 2020 Aug 25.
Chitosan oligosaccharides (COS) can elicit plant immunity and defence responses in rice plants, but exactly how this promotes plant growth remains largely unknown. Herein, we explored the effects of 0.5 mg/L COS on plant growth promotion in rice seedlings by measuring root and stem length, investigating biochemical factors in whole plants via proteomic analysis, and confirming upregulated and downregulated genes by real-time quantitative PCR. Pathway enrichment results showed that COS promoted root and stem growth, and stimulated metabolic (biosynthetic and catabolic processes) and photosynthesis in rice plants during the seedling stage. Expression levels of genes related to chlorophyll a-b binding, RNA binding, catabolic processes and calcium ion binding were upregulated following COS treatment. Furthermore, comparative analysis indicated that numerous proteins involved in the biosynthesis, metabolic (catabolic) processes and photosynthesis pathways were upregulated. The findings indicate that COS may upregulate calcium ion binding, photosynthesis, RNA binding, and catabolism proteins associated with plant growth during the rice seedling stage.
壳寡糖（COS）可以诱导水稻植物的免疫和防御反应，但它如何促进植物生长在很大程度上仍然未知。在此，我们通过测量根和茎的长度、通过蛋白质组学分析研究整个植物的生化因子，并通过实时定量 PCR 确认上调和下调的基因，来探索 0.5mg/L COS 对水稻幼苗生长促进的影响。途径富集结果表明，COS 促进了根和茎的生长，并在水稻幼苗阶段刺激了代谢（合成和分解代谢过程）和光合作用。用 COS 处理后，与叶绿素 a-b 结合、RNA 结合、分解代谢过程和钙离子结合相关的基因表达水平上调。此外，比较分析表明，许多与生物合成、代谢（分解代谢）过程和光合作用途径相关的蛋白质被上调。研究结果表明，COS 可能在水稻幼苗阶段上调与植物生长相关的钙离子结合、光合作用、RNA 结合和分解代谢蛋白。
