• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

壳寡糖处理感染南方水稻黑条矮缩病毒的水稻的无标记定量蛋白质组学分析。

Label-Free Quantitative Proteomic Analysis of Chitosan Oligosaccharide-Treated Rice Infected with Southern Rice Black-Streaked Dwarf Virus.

机构信息

State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering/Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China.

Hainan ZhengyeZhongnong High Techchnolngy Co., Ltd/National Joint Engineering Laboratory of marine biological pesticide discovery, Haikou 570206, China.

出版信息

Viruses. 2017 May 18;9(5):115. doi: 10.3390/v9050115.

DOI:10.3390/v9050115
PMID:28524115
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5454427/
Abstract

Southern rice black-streaked dwarf virus (SRBSDV) has spread from thesouth of China to the north of Vietnam in the past few years and severelyinfluenced rice production. Its long incubation period and early symptoms are not evident; thus, controlling it is difficult. Chitosan oligosaccharide (COS) is a green plant immunomodulator. Early studies showed that preventing and controlling SRBSDV have a certain effect and reduce disease infection rate, but its underlying controlling and preventing mechanism is unclear. In this study, label-free proteomics was used to analyze differentially expressed proteins in rice after COS treatment. The results showed that COS can up-regulate the plant defense-related proteins and down-regulate the protein expression levels of SRBSDV. Meanwhile, quantitative real-time PCR test results showed that COS can improve defense gene expression in rice. Moreover, COS can enhance the defense enzymatic activities of peroxidase, superoxide dismutase and catalase through mitogen-activated protein kinase signaling cascade pathway, and enhance the rice disease resistance.

摘要

南方水稻黑条矮缩病毒（SRBSDV）在过去几年中已从中国南方传播到越南北部，严重影响了水稻生产。其潜伏期长，初期症状不明显，因此难以控制。壳寡糖（COS）是一种绿色植物免疫调节剂。早期研究表明，预防和控制 SRBSDV 具有一定的效果，可以降低病害感染率，但其潜在的控制和预防机制尚不清楚。在这项研究中，使用无标记蛋白质组学分析了 COS 处理后水稻中差异表达的蛋白质。结果表明，COS 可以上调与植物防御相关的蛋白质，并下调 SRBSDV 的蛋白表达水平。同时，实时定量 PCR 测试结果表明，COS 可以提高水稻中防御基因的表达。此外，COS 可以通过丝裂原活化蛋白激酶信号级联途径增强过氧化物酶、超氧化物歧化酶和过氧化氢酶的防御酶活性，从而增强水稻的抗病性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/0efa4f27409d/viruses-09-00115-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/118308c4d049/viruses-09-00115-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/a951b6786500/viruses-09-00115-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/a16012a52e44/viruses-09-00115-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/84e7da7ebcf0/viruses-09-00115-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/e929944e1267/viruses-09-00115-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/32b5c402df47/viruses-09-00115-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/187626146f8f/viruses-09-00115-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/753e7bf68af0/viruses-09-00115-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/0efa4f27409d/viruses-09-00115-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/118308c4d049/viruses-09-00115-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/a951b6786500/viruses-09-00115-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/a16012a52e44/viruses-09-00115-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/84e7da7ebcf0/viruses-09-00115-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/e929944e1267/viruses-09-00115-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/32b5c402df47/viruses-09-00115-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/187626146f8f/viruses-09-00115-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/753e7bf68af0/viruses-09-00115-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e59e/5454427/0efa4f27409d/viruses-09-00115-g009.jpg

相似文献

1
Label-Free Quantitative Proteomic Analysis of Chitosan Oligosaccharide-Treated Rice Infected with Southern Rice Black-Streaked Dwarf Virus.壳寡糖处理感染南方水稻黑条矮缩病毒的水稻的无标记定量蛋白质组学分析。
Viruses. 2017 May 18;9(5):115. doi: 10.3390/v9050115.
2
Label-free quantitative proteomics analysis of Cytosinpeptidemycin responses in southern rice black-streaked dwarf virus-infected rice.无标记定量蛋白质组学分析南方水稻黑条矮缩病毒感染水稻中胞嘧啶肽霉素的反应。
Pestic Biochem Physiol. 2018 May;147:20-26. doi: 10.1016/j.pestbp.2017.06.005. Epub 2017 Jun 9.
3
Characteristics of siRNAs derived from Southern rice black-streaked dwarf virus in infected rice and their potential role in host gene regulation.南方水稻黑条矮缩病毒感染水稻后产生的小干扰RNA的特征及其在宿主基因调控中的潜在作用。
Virol J. 2017 Feb 10;14(1):27. doi: 10.1186/s12985-017-0699-3.
4
Evaluation of Rice Resistance to Southern Rice Black-Streaked Dwarf Virus and Rice Ragged Stunt Virus through Combined Field Tests, Quantitative Real-Time PCR, and Proteome Analysis.通过联合田间试验、定量实时聚合酶链反应和蛋白质组分析评估水稻对南方水稻黑条矮缩病毒和水稻锯齿叶矮缩病毒的抗性
Viruses. 2017 Feb 22;9(2):37. doi: 10.3390/v9020037.
5
Transcriptional changes of rice in response to rice black-streaked dwarf virus.水稻对水稻黑条矮缩病毒响应的转录变化
Gene. 2017 Sep 10;628:38-47. doi: 10.1016/j.gene.2017.07.015. Epub 2017 Jul 9.
6
Simultaneous detection and differentiation of Rice black streaked dwarf virus (RBSDV) and Southern rice black streaked dwarf virus (SRBSDV) by duplex real time RT-PCR.采用双重实时 RT-PCR 同时检测和区分水稻黑条矮缩病毒（RBSDV）和南方水稻黑条矮缩病毒（SRBSDV）。
Virol J. 2013 Jan 18;10:24. doi: 10.1186/1743-422X-10-24.
7
A novel method for transmitting southern rice black-streaked dwarf virus to rice without insect vector.一种无需昆虫介体就能将南方水稻黑条矮缩病毒传播至水稻的新方法。
Virol J. 2017 Aug 15;14(1):155. doi: 10.1186/s12985-017-0815-4.
8
Rice black-streaked dwarf virus P10 acts as either a synergistic or antagonistic determinant during superinfection with related or unrelated virus.水稻黑条矮缩病毒 P10 在与相关或不相关病毒的超感染过程中充当协同或拮抗决定因素。
Mol Plant Pathol. 2019 May;20(5):641-655. doi: 10.1111/mpp.12782. Epub 2019 Feb 14.
9
Quantification of southern rice black streaked dwarf virus and rice black streaked dwarf virus in the organs of their vector and nonvector insect over time.随时间推移定量分析介体昆虫及其非介体昆虫器官中的南方水稻黑条矮缩病毒和水稻黑条矮缩病毒。
Virus Res. 2015 Oct 2;208:146-55. doi: 10.1016/j.virusres.2015.06.015. Epub 2015 Jun 23.
10
Evaluation of reference genes and expression of key genes involved in the isoprenoid metabolic pathway of rice leaves after infection by the Southern rice black-streaked dwarf virus.南方水稻黑条矮缩病毒侵染后水稻叶片类异戊二烯代谢途径关键基因表达及其参照基因的评价。
Mol Biol Rep. 2019 Aug;46(4):3945-3953. doi: 10.1007/s11033-019-04841-4. Epub 2019 Apr 29.

引用本文的文献

1
The Emerging Role of Omics-Based Approaches in Plant Virology.基于组学方法在植物病毒学中的新兴作用。
Viruses. 2025 Jul 15;17(7):986. doi: 10.3390/v17070986.
2
The Past, Present, and Future of Plant Activators Targeting the Salicylic Acid Signaling Pathway.植物激活剂靶向水杨酸信号通路的过去、现在和未来。
Genes (Basel). 2024 Sep 23;15(9):1237. doi: 10.3390/genes15091237.
3
Physiological and Proteome Analysis of the Effects of Chitosan Oligosaccharides on Salt Tolerance of Rice Seedlings.壳寡糖对水稻幼苗耐盐性影响的生理及蛋白质组学分析。

本文引用的文献

1
The STRING database in 2017: quality-controlled protein-protein association networks, made broadly accessible.2017年的STRING数据库：质量可控的蛋白质-蛋白质相互作用网络，广泛可用。
Nucleic Acids Res. 2017 Jan 4;45(D1):D362-D368. doi: 10.1093/nar/gkw937. Epub 2016 Oct 18.
2
Chitosan oligosaccharide induces resistance to Tobacco mosaic virus in Arabidopsis via the salicylic acid-mediated signalling pathway.壳寡糖通过水杨酸介导的信号通路诱导拟南芥对烟草花叶病毒产生抗性。
Sci Rep. 2016 May 18;6:26144. doi: 10.1038/srep26144.
3
Expression of TaWRKY44, a wheat WRKY gene, in transgenic tobacco confers multiple abiotic stress tolerances.
Int J Mol Sci. 2024 May 29;25(11):5953. doi: 10.3390/ijms25115953.
4
Single-cell and spatial RNA sequencing reveal the spatiotemporal trajectories of fruit senescence.单细胞和空间 RNA 测序揭示了果实衰老的时空轨迹。
Nat Commun. 2024 Apr 10;15(1):3108. doi: 10.1038/s41467-024-47329-x.
5
Molecular insights into the responses of barley to yellow mosaic disease through transcriptome analysis.通过转录组分析揭示大麦对黄花叶病响应的分子机制。
BMC Plant Biol. 2023 May 19;23(1):267. doi: 10.1186/s12870-023-04276-x.
6
A Proteomics Insight into Advancements in the Rice-Microbe Interaction.水稻与微生物相互作用进展的蛋白质组学洞察
Plants (Basel). 2023 Feb 28;12(5):1079. doi: 10.3390/plants12051079.
7
Plant Protection against Viruses: An Integrated Review of Plant Immunity Agents.植物抗病毒保护：植物免疫剂的综合评述。
Int J Mol Sci. 2023 Feb 23;24(5):4453. doi: 10.3390/ijms24054453.
8
Chitinase-Assisted Bioconversion of Chitinous Waste for Development of Value-Added Chito-Oligosaccharides Products.几丁质酶辅助甲壳质废料生物转化以开发增值壳寡糖产品
Biology (Basel). 2023 Jan 5;12(1):87. doi: 10.3390/biology12010087.
9
Quality assessment and nutrient uptake and utilization in Luohan pine (Podocarpus macrophyllus) seedlings raised by chitosan spraying in varied LED spectra.不同 LED 光谱下壳聚糖喷雾对罗汉松（Podocarpus macrophyllus）幼苗质量评估及养分吸收和利用的影响。
PLoS One. 2022 Apr 28;17(4):e0267632. doi: 10.1371/journal.pone.0267632. eCollection 2022.
10
The Potential of Using Chitosan on Cereal Crops in the Face of Climate Change.面对气候变化，壳聚糖在谷类作物上的应用潜力
Plants (Basel). 2021 Jun 7;10(6):1160. doi: 10.3390/plants10061160.
小麦WRKY基因TaWRKY44在转基因烟草中的表达赋予多种非生物胁迫耐受性。
Front Plant Sci. 2015 Aug 11;6:615. doi: 10.3389/fpls.2015.00615. eCollection 2015.
4
P5-2 of rice black-streaked dwarf virus is a non-structural protein targeted to chloroplasts.水稻黑条矮缩病毒的P5-2是一种靶向叶绿体的非结构蛋白。
Arch Virol. 2015 May;160(5):1211-7. doi: 10.1007/s00705-015-2382-6. Epub 2015 Mar 10.
5
Chitin and chitosan preparation from marine sources. Structure, properties and applications.源于海洋资源的几丁质和壳聚糖制备。结构、性质及应用。
Mar Drugs. 2015 Mar 2;13(3):1133-74. doi: 10.3390/md13031133.
6
Characterization of homologous and heterologous interactions between viroplasm proteins P6 and P9-1 of the fijivirus southern rice black-streaked dwarf virus.水稻黑条矮缩病毒斐济病毒属病毒粒体蛋白P6和P9-1之间同源和异源相互作用的特征分析
Arch Virol. 2015 Feb;160(2):453-7. doi: 10.1007/s00705-014-2268-z. Epub 2014 Nov 7.
7
Isolation from Rubus cell-suspension cultures of a lectin specific for glucosamine oligomers.从悬液培养的悬钩子细胞中分离出一种对氨基葡萄糖低聚物特异的凝集素。
Planta. 1991 Apr;184(1):8-13. doi: 10.1007/BF00208229.
8
Non-structural protein P6 encoded by rice black-streaked dwarf virus is recruited to viral inclusion bodies by binding to the viroplasm matrix protein P9-1.由水稻黑条矮缩病毒编码的非结构蛋白 P6 通过与病毒包涵体基质蛋白 P9-1 结合而被招募到病毒包涵体中。
J Gen Virol. 2013 Aug;94(Pt 8):1908-1916. doi: 10.1099/vir.0.051698-0. Epub 2013 May 1.
9
Heat exposure alters the expression of SOD, POD, APX and CAT isozymes and mitigates low cadmium toxicity in seedlings of sensitive and tolerant rice cultivars.热暴露改变了 SOD、POD、APX 和 CAT 同工酶的表达，减轻了敏感和耐受水稻品种幼苗的低镉毒性。
Plant Physiol Biochem. 2012 Aug;57:106-13. doi: 10.1016/j.plaphy.2012.05.007. Epub 2012 May 23.
10
KEGG for integration and interpretation of large-scale molecular data sets.KEGG 用于整合和解释大规模分子数据集。
Nucleic Acids Res. 2012 Jan;40(Database issue):D109-14. doi: 10.1093/nar/gkr988. Epub 2011 Nov 10.