Biometals and Biological Chemistry, Institut de Chimie, UMR 7177, CNRS-Université de Strasbourg, 4 rue Blaise Pascal, 67 000 Strasbourg, France.
Membrane Biophysics and NMR, Institut de Chimie, UMR 7177, CNRS-Université de Strasbourg, 4 rue Blaise Pascal, 67 000 Strasbourg, France.
J Inorg Biochem. 2020 Dec;213:111255. doi: 10.1016/j.jinorgbio.2020.111255. Epub 2020 Sep 11.
Depending on the coordination, copper ions can have a very high activity in catalyzing the production of reactive oxygen species. Thus interest arose in increasing the activity of antimicrobial peptides (AMPs) by equipping them with a Cu-binding unit. Several examples, native and engineered, have been investigated with the motif Xxx-Zzz-His, called Amino Terminal Cu(II)- and Ni(II)-binding (ATCUN) motif. Here we investigate a short AMP that was equipped either with Xxx-Zzz-His or Xxx-His. Xxx-His is a shorter motif and yields a more redox active copper complex. The control AMP, Xxx-His-AMP and Xxx-Zzz-His-AMP were investigated toward Cu-binding, Reactive Oxygen Species (ROS) production and antimicrobial activity in E. coli. The data indicate that these Cu-binding motifs have very limited impact on antimicrobial activity and low ROS production capability.
根据协调情况，铜离子在催化活性氧物质的产生方面具有非常高的活性。因此，人们开始通过为抗菌肽（AMP）配备铜结合单元来提高其活性。已经研究了几种具有 Xxx-Zzz-His 基序的天然和工程实例，该基序称为氨基末端 Cu(II)和 Ni(II)结合（ATCUN）基序。在这里，我们研究了一种经过修饰的短 AMP，它配备了 Xxx-Zzz-His 或 Xxx-His。Xxx-His 是一个更短的基序，产生更具氧化还原活性的铜配合物。对照 AMP，Xxx-His-AMP 和 Xxx-Zzz-His-AMP 被研究了其对铜结合、活性氧物质（ROS）产生和大肠埃希菌中的抗菌活性的影响。数据表明，这些铜结合基序对抗菌活性和低 ROS 产生能力的影响非常有限。
