Iannuzzi Alessandra, Della Valle Giovanni, Russo Marco, Longobardi Valentina, Albero Giuseppe, De Canditiis Carolina, Kosior Michal Andrzej, Pistucci Ramona, Gasparrini Bianca
National Research Council (CNR), ISPAAM, Laboratory of Animal Cytogenetics and Genomics, Via Argine, 1085, 80147, Naples, Italy.
Department of Veterinary Medicine and Animal Production, Federico II University of Naples, Via F. Delpino 1, 80137, Naples, Italy.
Theriogenology. 2020 Dec;158:227-232. doi: 10.1016/j.theriogenology.2020.09.019. Epub 2020 Sep 15.
The study aimed to evaluate if the sperm telomere length can be considered as a new biomarker for sperm quality in bulls. Sperm Telomere Length was evaluated by Monochrome Multiplex Quantitative PCR in group A (n = 8) and group B (n = 8) bulls, classified according to standard semen analysis. Also, this parameter was measured before and after Percoll gradient separation within bulls that produced semen of satisfactory quality. Sperm telomere length, measured as T/S ratio (average ratio of telomere repeats copy number to a single copy gene), was higher in group A than in group B bulls (0.77 ± 0.03 vs 0.43 ± 0.06; P < 0.01). Sperm telomere length was positively correlated with motility, viability and membrane integrity, and it was negatively correlated with sperm anomalies. Furthermore, Percoll gradient selected sperms with higher T/S ratio than unselected sperms (1.19 ± 0.02 vs 0.67 ± 0.03). These results suggest that sperm telomere length can be used as a new marker of bovine semen quality.
该研究旨在评估精子端粒长度是否可被视为公牛精子质量的一种新生物标志物。通过单色多重定量PCR对A组（n = 8）和B组（n = 8）公牛的精子端粒长度进行评估，这两组公牛是根据标准精液分析分类的。此外，在产生质量合格精液的公牛中，在Percoll梯度分离前后测量该参数。以T/S比值（端粒重复拷贝数与单拷贝基因的平均比值）衡量的精子端粒长度，A组公牛高于B组公牛（0.77±0.03对0.43±0.06；P<0.01）。精子端粒长度与活力、存活率和膜完整性呈正相关，与精子异常呈负相关。此外，Percoll梯度选择的精子T/S比值高于未选择的精子（1.19±0.02对0.67±0.03）。这些结果表明，精子端粒长度可作为牛精液质量的一种新标志物。
