Department of Cardiothoracic Surgery, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200437, China.
Institute of Clinical Immunology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200437, China.
Chin J Integr Med. 2021 Feb;27(2):98-105. doi: 10.1007/s11655-020-3158-8. Epub 2020 Sep 26.
To investigate the action mechanisms of electroacupuncture (EA) on postoperative immunosuppression.
Male C57BL/6 mice (5-7 weeks old) were randomly divided into: the sham injury group, the surgical trauma stressed group, the EA group [surgery + 2/100 Hz EA at Neiguan (PC 6)], and the EA+ Nal (surgery + EA + intraperitoneal injection of naloxone). Abdominal surgical trauma stress mice model was established. EA was performed on bilateral PC 6 acupoints by an EA apparatus (2/100 Hz) for 20 min once a day for 3 days. The mRNA expressions of MOR, DOR, and KOR in thymus and L3-L5 dorsal root ganglions (DRG) were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and the protein expressions of MOR, DOR, and KOR in thymus were measured by Western blot. Flow cytometry assay was used to detect the levels of T lymphocyte subtypes in the peripheral blood.
Surgical trauma induced decreased the mRNA expression level of MOR in both thymus (P<0.01) and L3`-L5 DRGs (P<0.05). Moreover, EA treatment not only significantly attenuated the MOR protein and mRNA expression in the thymus (both P<0.05), but also markedly increased expression of DOR and KOR opioid receptor in thymus (P<0.01). However, the mRNA expressions of opioid receptors were not regulated by EA in the DRG (all P>0.05). Furthermore, T lymphocyte population of CD3 and CD4 was decreased in the peripheral blood after surgical trauma (both P<0.01). EA treatment can significantly elevate the population of CD (P<0.01), CD (P<0.05) and CD T cells (P<0.01). Intraperitoneal injection of the non-selective opioid receptor antagonist naloxone blocked the up-regulation of T lymphocytes by EA.
EA may improve postoperative immunosuppression through the peripheral opioid system.
探讨电针对术后免疫抑制的作用机制。
雄性 C57BL/6 小鼠（5-7 周龄）随机分为：假损伤组、手术创伤应激组、电针组[手术+2/100 Hz 电针内关（PC6）]和电针+Nal 组（手术+电针+腹腔注射纳洛酮）。建立腹部手术创伤应激小鼠模型。采用电针仪（2/100 Hz）对双侧 PC6 穴位进行电针治疗，每天 1 次，每次 20 min，连续 3 天。采用实时定量聚合酶链反应（qRT-PCR）检测胸腺和 L3`-L5 背根神经节（DRG）中 MOR、DOR 和 KOR 的 mRNA 表达，Western blot 法检测胸腺中 MOR、DOR 和 KOR 的蛋白表达。采用流式细胞术检测外周血 T 淋巴细胞亚群水平。
手术创伤导致胸腺（P<0.01）和 L3`-L5 DRG（P<0.05）中 MOR 的 mRNA 表达水平降低。此外，电针治疗不仅显著减弱了胸腺中 MOR 蛋白和 mRNA 的表达（均 P<0.05），而且明显增加了胸腺中 DOR 和 KOR 阿片受体的表达（均 P<0.01）。然而，电针在 DRG 中并没有调节阿片受体的 mRNA 表达（均 P>0.05）。此外，手术创伤后外周血中 CD3 和 CD4 阳性 T 淋巴细胞群减少（均 P<0.01）。电针治疗可显著提高 CD（P<0.01）、CD（P<0.05）和 CD T 细胞群（P<0.01）。腹腔注射非选择性阿片受体拮抗剂纳洛酮阻断了电针对 T 淋巴细胞的上调作用。
电针可能通过外周阿片系统改善术后免疫抑制。
