Bernard J M, Gras-Masse H, Drobecq H, Tartar A, Lefrancier P, Hosmalin A, Carelli C, Chedid L
Int J Pept Protein Res. 1987 Apr;29(4):455-63. doi: 10.1111/j.1399-3011.1987.tb02271.x.
Two glycopeptides associating the aminoacid sequence of LH-RH with MDP were prepared, using a Lys residue as a linker. These conjugates, N alpha MDP N epsilon (LH-RH)-Lys and N alpha MDP N epsilon (LH-RH)-Lys-NH2 obtained by condensation of fragments were synthesized by liquid as well as solid phase methods. Both compounds were able to induce anti LH-RH antibodies and immunological castration. They retained the immuno-adjuvant activity of MDP. Such antigen-adjuvant constructs, devoid of carrier and obtained by chemically defined and reproducible synthetic methods could offer suitable tools for structure-activity relationship studies aiming at defining synthetic vaccines.
