Mahmoud Hany, Ammar Hatem, El Rashidy Amr, Ali Amal H, Hefny Hesham M, Mounir Amr
Sohag Faculty of Medicine, Ophthalmology Department, Sohag University, Sohag, Egypt.
Tropical Medicine, Sohag Tropical Medicine Hospital, Sohag, Egypt.
Clin Ophthalmol. 2020 Sep 15;14:2701-2708. doi: 10.2147/OPTH.S270006. eCollection 2020.
To assess SARS-CoV-2 virus in conjunctival tears and secretions of positive confirmed COVID-19 patients.
A case series study that included 28 positive COVID-19 patients confirmed with nasopharyngeal swab in the period 18-28 May 2020 at Sohag Tropical Medicine Hospital. Tears and conjunctival secretions of these confirmed positive cases were collected with disposable sampling swabs at interval of 3 days after admission due to respiratory symptoms. They were examined for the presence of SARS-CoV-2 by reverse transcription-polymerase chain reaction (RT-PCR) assay.
Thirteen (46.43%) patients were stable, 4 (14.28%) patients suffered from dyspnea, 3 (10.72%) patients suffered from high fever, 5 (17.85%) patients suffered from cough, and 3 (10.72%) patients were on mechanical ventilation. Ten (35.71%) patients suffered from conjunctivitis. Tear and conjunctival swabs were positive in 8 (28.57%) patients, while other patients' swabs were negative (71.43%). Out of 10 patients with conjunctival manifestations, 3 patients had SARS-CoV-2 in their conjunctiva using (RT-PCR) test. Out of the 18 patients with no conjunctival manifestations, 5 patients had positive SARS-CoV-2 in their conjunctiva using (RT-PCR) test.
The SARS-CoV-2 virus could be found in tears and conjunctival secretions in SARS-CoV-2 patients with or without conjunctivitis.
评估新型冠状病毒肺炎（COVID-19）确诊阳性患者结膜泪液及分泌物中的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）病毒。
一项病例系列研究，纳入了2020年5月18日至28日在索哈格热带医学医院经鼻咽拭子确诊的28例COVID-19阳性患者。这些确诊阳性病例因呼吸道症状入院后，每隔3天用一次性采样拭子采集泪液和结膜分泌物。通过逆转录聚合酶链反应（RT-PCR）检测法检测其中是否存在SARS-CoV-2病毒。
13例（46.43%）患者病情稳定，4例（14.28%）患者出现呼吸困难，3例（10.72%）患者出现高热，5例（17.85%）患者出现咳嗽，3例（10.72%）患者接受机械通气。10例（35.71%）患者患有结膜炎。8例（28.57%）患者的泪液和结膜拭子检测呈阳性，其他患者的拭子检测为阴性（71.43%）。在10例有结膜表现的患者中，3例通过（RT-PCR）检测发现结膜中有SARS-CoV-2病毒。在18例无结膜表现的患者中，5例通过（RT-PCR）检测发现结膜中有SARS-CoV-2病毒呈阳性。
在有或无结膜炎的SARS-CoV-2患者的泪液和结膜分泌物中均可发现SARS-CoV-2病毒。
