Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
Division of Children and Women Health, Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden.
Front Immunol. 2020 Aug 24;11:1916. doi: 10.3389/fimmu.2020.01916. eCollection 2020.
Despite that autoimmune diseases share similar immunogenetic mechanisms, studies comparing the protein composition in peripheral blood from patients with autoimmune endocrine diseases are limited. In this study, we applied proximity extension assay to measure proteins related to signaling and interactions within the immune system in peripheral blood from patients with new-onset (N-T1D) and long-standing (L-T1D) type 1 diabetes, Hashimoto's thyroiditis (HT), Graves' disease (GD), and autoimmune Addison's disease in addition to healthy controls (HC). Proteins in plasma and supernatants from cultured PBMC were measured by using a 92-plex Olink® INFLAMMATION panel. Soluble CDCP1 was more abundant in plasma from patients with N-T1D, L-T1D, HT, and GD than in HC. The L-T1D and HT groups had elevated plasma levels of SLAMF1 compared with HC. Patients and HC could not be distinguished by their protein composition in PBMC supernatants. The high-throughput multiplex technology enabled us to detect two low-abundant proteins that have been gradually connected to autoimmune diseases. Our study provides novel associations between CDCP1, SLAMF1, and autoimmune endocrine diseases, which might reflect a higher degree of inflammation and lymphocyte activation.
尽管自身免疫性疾病具有相似的免疫遗传机制，但比较自身免疫性内分泌疾病患者外周血中蛋白质组成的研究有限。在这项研究中，我们应用邻近延伸分析测定法来测量新发病（N-T1D）和长期（L-T1D）1 型糖尿病、桥本甲状腺炎（HT）、格雷夫斯病（GD）和自身免疫性艾迪生病患者外周血中与免疫系统信号转导和相互作用相关的蛋白质，此外还包括健康对照（HC）。使用 92 个斑点 Olink®INFLAMMATION 面板测量血浆和培养 PBMC 上清液中的蛋白质。与 HC 相比，N-T1D、L-T1D、HT 和 GD 患者的血浆中可溶性 CDCP1 更为丰富。与 HC 相比，L-T1D 和 HT 组的 SLAMF1 血浆水平升高。患者和 HC 不能通过 PBMC 上清液中的蛋白质组成来区分。高通量多重技术使我们能够检测到两种低丰度蛋白质，它们逐渐与自身免疫性疾病有关。我们的研究为 CDCP1、SLAMF1 与自身免疫性内分泌疾病之间的新关联提供了依据，这可能反映了更高程度的炎症和淋巴细胞激活。
