Bakuła Zofia, Gromadka Robert, Gawor Jan, Siedlecki Paweł, Pomorski Jan J, Maciszewski Kacper, Gromadka Agnieszka, Karnkowska Anna, Jagielski Tomasz
Department of Medical Microbiology, Faculty of Biology, Institute of Microbiology, University of Warsaw, Warsaw, Poland.
DNA Sequencing and Oligonucleotides Synthesis Laboratory at the Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
Front Plant Sci. 2020 Sep 1;11:1296. doi: 10.3389/fpls.2020.01296. eCollection 2020.
Of the genus, has the highest clinical significance in humans. However, neither nuclear nor organellar genomes of this species were sequenced until now. The hitherto determined and analyzed mitochondrial and plastid genomes of the alleged species belong in fact to another species, recently named e. This study provides a first insight into the organellar genomes of a true (type strain ATCC 16529). The mitochondrion had a 53.8-kb genome, which was considerably larger than that of (formerly gen. 1) and (formerly gen. 2), yet similarly functional, with the differences in size attributable to a higher number of introns and the presence of extra unique putative genes. The 48-kb plastid genome of , compared to autotrophic Trebouxiophyceae, was highly reduced due to the elimination of the photosynthesis-related genes. The gene content of the plastid genome of was, however, very similar to other colorless species. Plastid genome-based phylogeny reinforced the polyphyly of the genus , with and branching within clades of species. Phylogenetic reconstruction also confirmed the close relationship of and , which is reflected in the synteny of their organellar genomes. Interestingly, the entire set of genes was lost in plastid genome while being preserved in .
在该属中，[具体物种名称]对人类具有最高的临床意义。然而，该物种的核基因组和细胞器基因组此前均未被测序。迄今为止所测定和分析的所谓[疑似物种名称]的线粒体和质体基因组实际上属于另一个物种，该物种最近被命名为[新物种名称]。本研究首次深入探究了真正的[具体物种名称]（模式菌株ATCC 16529）的细胞器基因组。该线粒体具有一个53.8 kb的基因组，这比[相关物种1名称]（以前的[相关属1名称]）和[相关物种2名称]（以前的[相关属2名称]）的基因组大得多，但功能类似，大小差异归因于内含子数量更多以及存在额外的独特假定基因。与自养的绿球藻纲相比，[具体物种名称]的48 kb质体基因组由于光合作用相关基因的缺失而高度简化。然而，[具体物种名称]质体基因组的基因内容与其他无色[相关物种名称]非常相似。基于质体基因组的系统发育强化了[相关属名称]属的多系性，[具体物种1名称]和[具体物种2名称]在[相关物种名称]物种的分支内分支。系统发育重建也证实了[具体物种1名称]和[具体物种2名称]的密切关系，这在它们细胞器基因组的同线性中得到体现。有趣的是，[具体物种名称]质体基因组中整套[具体基因名称]基因丢失了，而在[具体物种名称]中得以保留。
