Zhuo Minglei, An Tongtong, Zhang Chaoting, Wang Ziping
Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Thoracic Oncology, Peking University Cancer Hospital & Institute, Beijing, China.
Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Laboratory of Biochemistry and Molecular Biology, Peking University Cancer Hospital & Institute, Beijing, China.
Front Oncol. 2020 Aug 24;10:1584. doi: 10.3389/fonc.2020.01584. eCollection 2020.
The functional role of lung microbiota has attracted an accumulating attention recently, but the profile and functional role of the lung microbiota in patients with lung cancer remained largely unknown.
To evaluate the association of the microbiota with lung cancer, we performed comparative analysis of the lung microbiota using 16S rRNA amplicon sequencing approach in the paired bronchoalveolar lavage fluid (BALF) samples (paired samples from cancerous lung and the contralateral non-cancerous lung) from 50 cancer patients with unilateral lobar masses.
We found that the relative abundance of phylum Tenericutes, its class Mollicutes, its order Entomoplasmatales, its family Spiroplasmataceae, and its genus was significantly increased in cancerous lung, but the relative abundance of phylum Bacteroidetes, its class Bacteroidia, and its order Bacteroidales was significantly decreased in cancerous lung. In addition, the relative abundance of family Leuconostocaceae and its genus was significantly increased in cancerous lung.
Our findings provide insights into a change of lung microbiota community associated with the development of lung cancer.
肺部微生物群的功能作用近来受到越来越多的关注，但肺癌患者肺部微生物群的特征和功能作用仍 largely 未知。
为评估微生物群与肺癌的关联，我们采用 16S rRNA 扩增子测序方法，对 50 例单侧肺叶肿块癌症患者的配对支气管肺泡灌洗（BALF）样本（来自癌肺和对侧非癌肺的配对样本）中的肺部微生物群进行了比较分析。
我们发现，柔膜菌门及其柔膜菌纲、虫原体目、螺旋体科及其属在癌肺中的相对丰度显著增加，但拟杆菌门及其拟杆菌纲和拟杆菌目在癌肺中的相对丰度显著降低。此外，明串珠菌科及其属在癌肺中的相对丰度显著增加。
我们的研究结果为与肺癌发生相关的肺部微生物群群落变化提供了见解。
