Hoffenbach A, Diioro L, Bach M A
Int J Lepr Other Mycobact Dis. 1987 Jun;55(2):305-15.
Mice of the C57BL/6 strain were injected either subcutaneously (s.c.) in the foot pad or intravenously (i.v.) with 10(5) or 10(8) Mycobacterium lepraemurium (MLM). Anti-MLM sonicate total immunoglobulin (Ig), IgM, and IgG antibody production was followed during the course of the infection. The kinetics of appearance and the magnitude of anti-MLM antibodies were found to be related to the size and route of inoculation. The i.v. route induced earlier and higher amounts of anti-MLM antibodies than did the s.c. route. In i.v.-infected mice, a relatively predominant IgM response to MLM was observed, while a relatively higher IgG response was seen in s.c.-infected mice. IgM antibody level was found to increase sharply with the bacterial load as assessed in the spleens of mice with i.v.-disseminated infection; whereas a slow progression of both IgM and IgG levels was noted with time in s.c.-infected mice.
将C57BL/6品系的小鼠通过足垫皮下注射（s.c.）或静脉注射（i.v.）10⁵或10⁸鼠麻风杆菌（MLM）。在感染过程中追踪抗MLM超声裂解物总免疫球蛋白（Ig）、IgM和IgG抗体的产生情况。发现抗MLM抗体出现的动力学及抗体量与接种的菌量和途径有关。静脉注射途径比皮下注射途径诱导产生抗MLM抗体的时间更早且量更多。在静脉感染的小鼠中，观察到对MLM相对占优势的IgM反应，而在皮下感染的小鼠中则观察到相对较高的IgG反应。发现静脉播散感染小鼠脾脏中的IgM抗体水平随细菌载量急剧增加；而皮下感染小鼠中IgM和IgG水平随时间缓慢上升。
