Lasareishvili Besarion, Shi Hang, Wang Xunhao, Hillstead Kyle D, Tediashvili Marina, Jaiani Ekaterine, Tarabara Volodymyr V
School of Engineering and Technologies, Agricultural University of Georgia, Kakha Bendukidze University Campus, Tbilisi, Georgia.
Department of Civil and Environmental Engineering, Michigan State University, East Lansing, Michigan, USA.
Biotechnol Prog. 2021 Jan;37(1):e3080. doi: 10.1002/btpr.3080. Epub 2020 Oct 8.
A simple model is developed to describe the instantaneous (r ) and cumulative (R ) recovery of viruses from water during sample concentration by tangential flow filtration in the regime of constant water recovery, r. A figure of merit, M = r r, is proposed as an aggregate performance metric that captures both the efficiency of virus recovery and the speed of sample concentration. We derive an expression for virus concentration in the sample as a function of filtration time with the rate-normalized virus loss, , as a parameter. A practically relevant case is considered when the rate of virus loss is proportional to the permeation-driven mass flux of viruses to the membrane: . In this scenario, the instantaneous recovery is constant, the cumulative recovery is decreasing as a power function of time, , η mediates the trade-off between r and r , and M is maximized at . The proposed model can guide the design of the sample concentration process and serve as a framework for quantification and interlaboratory comparison of experimental data on virus recovery.
开发了一个简单模型来描述在恒定水回收率r的条件下，通过切向流过滤进行样品浓缩时，水中病毒的瞬时回收率（r）和累积回收率（R）。提出了一个品质因数M = r r，作为一个综合性能指标，它既体现了病毒回收效率，又体现了样品浓缩速度。我们推导了样品中病毒浓度随过滤时间变化的表达式，其中以速率归一化的病毒损失 作为参数。考虑了一种实际相关的情况，即病毒损失速率与病毒向膜的渗透驱动质量通量成正比： 。在这种情况下，瞬时回收率是恒定的，累积回收率随时间呈幂函数下降， ，η调节r和r 之间的权衡，并且M在 时达到最大值。所提出的模型可以指导样品浓缩过程的设计，并作为对病毒回收实验数据进行量化和实验室间比较的框架。
