Department of Pathology and Laboratory Medicine Jordan University of Science and Technology Irbid, Jordan.
Department of Public Health, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.
Asian Pac J Cancer Prev. 2020 Sep 1;21(9):2783-2791. doi: 10.31557/APJCP.2020.21.9.2783.
In this study, we aimed to explore the relationship between five selected proinflammatory and immune-mediated genes (TNF rs1800629G>A, rs361525G>A, rs1799964T>C, LTA rs1800683G>A, rs909253A>G, TNFAIP8 rs1042541C>T, LEPR rs1327118G>C, and LEP rs2167270G>A) and the risk and overall survival of DLBCL patients within the Jordanian Arab population.
One hundred twenty-five patients (125) diagnosed with DLBCL at the King Abdullah University Hospital (KAUH) between 2013 and 2018 and 238 healthy cancer-free control subjects with similar geographic and ethnic backgrounds to the patients were included in the study. Genomic DNA was extracted from the formalin-fixed paraffin-embedded tissues of the subjects and from peripheral blood samples of the controls. The Sequenom MassARRAY® sequencer system (iPLEX GOLD) was used. The analyses included assessments of population variability and survival.
Our study showed significant differences in the distribution of the studied polymorphisms of DLBCL between the patients and controls for TNF rs1800629G>A, LTA rs909253 G>A and LEP rs2167270 G>A. TNF rs1800629G>A (p = 0.01), in which the G allele harbors a higher risk of DLBCL (GG and GA genotypes when compared with AA genotype) (p = 0.044). The LTA rs909253 A>G polymorphism is associated with a higher risk of DLBCL in the allelic model (p = .004). LEP rs2167270 G>A polymorphism is associated with a decreased risk of DLBCL in the recessive mode models (p = .03). Subjects with the dominant model for TNF-a rs1799964 (TT genotype in comparison with the combined TT/TC genotype) and patients with the homozygous genotype (GG) of rs361525 have better overall survival rates.
Our results confirmed the diversity and the heterogeneity of the disease. Although the study has a limitation because of its relatively small size, it clearly emphasizes the significance of ancestry and genetic composition as the determinants of DLBCL risk and behavior.
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本研究旨在探讨五个选定的促炎和免疫介导基因（TNF rs1800629G>A、rs361525G>A、rs1799964T>C、LTA rs1800683G>A、rs909253A>G、TNFAIP8 rs1042541C>T、LEPR rs1327118G>C 和 LEP rs2167270G>A）与约旦阿拉伯人群中弥漫性大 B 细胞淋巴瘤（DLBCL）患者的风险和总生存之间的关系。
本研究纳入了 2013 年至 2018 年在阿卜杜拉国王大学医院（KAUH）诊断为 DLBCL 的 125 名患者（125 例）和 238 名具有相似地理和种族背景的无癌症健康对照者。从受试者的福尔马林固定石蜡包埋组织和对照者的外周血样本中提取基因组 DNA。采用 Sequenom MassARRAY®测序仪（iPLEX GOLD）进行分析。分析包括人群变异和生存评估。
我们的研究表明，TNF rs1800629G>A、LTA rs909253 G>A 和 LEP rs2167270 G>A 等 DLBCL 研究多态性在患者和对照组之间的分布存在显著差异。TNF rs1800629G>A（p=0.01）中，G 等位基因增加了 DLBCL 的风险（与 AA 基因型相比，GG 和 GA 基因型）（p=0.044）。LTA rs909253 A>G 多态性与 DLBCL 的等位基因模型相关（p=0.004）。LEP rs2167270 G>A 多态性与隐性模式模型中 DLBCL 的风险降低相关（p=0.03）。TNF-α rs1799964 的显性模型（TT 基因型与 TT/TC 基因型的组合相比）和 rs361525 的纯合基因型（GG）的受试者具有更好的总生存率。
我们的结果证实了疾病的多样性和异质性。尽管由于样本量相对较小，研究存在一定局限性，但它清楚地强调了种族和遗传组成作为 DLBCL 风险和行为决定因素的重要性。
