Reusser F
Biochemistry. 1977 Jul 26;16(15):3406-12. doi: 10.1021/bi00634a018.
The two peptide-like antibiotics ficellomycin and feldamycin impair semiconservative DNA replication but not DNA repair synthesis in bacteria. Specifically both antibiotics cause the accumulation of a 34S DNA species in toluenized Escherichia coli cells which lacks the capability of being integrated into larger DNA pieces and eventually the complete bacterial chromosome. Novobiocin, a known inhibitor of replicative DNA synthesis, was investigated for comparative purposes. The action of this latter antibiotic differs from the ones exerted by ficellomycin and feldamycin in the novobiocin appears to block an event associated with the initiation of Okazaki fragments. The fact that novobiocin impairs DNA gyrase suggests that this enzyme plays an essential role during the initiation of Okazaki pieces.
两种肽类抗生素纤维霉素和费氏霉素会损害细菌中的半保留DNA复制，但不影响DNA修复合成。具体而言，这两种抗生素都会导致在经甲苯处理的大肠杆菌细胞中积累一种34S DNA物种，该细胞缺乏整合到更大DNA片段并最终形成完整细菌染色体的能力。为了进行比较，研究了已知的复制性DNA合成抑制剂新生霉素。后一种抗生素的作用与纤维霉素和费氏霉素的作用不同，新生霉素似乎阻断了与冈崎片段起始相关的事件。新生霉素会损害DNA促旋酶，这一事实表明该酶在冈崎片段起始过程中起着至关重要的作用。
