Fu Yanzhao, Huang Li, Zhao Shaojing, Xing Xuejian, Lan Minhuan, Song Xiangzhi
Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China; Shenzhen Research Institute of Central South University, Shenzhen 518057, PR China.
Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Feb 5;246:118947. doi: 10.1016/j.saa.2020.118947. Epub 2020 Sep 17.
A carbon dot (CD)-based fluorometric probe for oxytetracycline (OTC) detection utilizing a Förster resonance energy transfer (FRET) mechanism was firstly developed. Upon addition of OTC, the blue fluorescence of the CDs peaked at 405 nm was quickly quenched, with a new fluorescence peaked at 505 nm attributed to OTC was observed within 30 s. A visual fluorescent color change of the CDs solution from blue to green was discerned under a 365 nm UV light irradiation. The CDs displayed a high sensitivity and selectivity toward OTC with a low detection limit of 0.41 μM. Furthermore, the probe was applied to detect OTC in water, milk, and pork samples with a satisfied recovery.
首次开发了一种基于碳点（CD）的荧光探针，用于利用Förster共振能量转移（FRET）机制检测土霉素（OTC）。加入OTC后，CDs在405nm处的蓝色荧光迅速猝灭，30s内观察到归因于OTC的在505nm处有新的荧光峰。在365nm紫外光照射下，可辨别出CDs溶液从蓝色到绿色的视觉荧光颜色变化。CDs对OTC表现出高灵敏度和选择性，检测限低至0.41μM。此外，该探针应用于水、牛奶和猪肉样品中OTC的检测，回收率令人满意。
