Lv Linlin, Luo Weiwei, Diao Quanping
School of Chemistry and Life Science, Anshan Normal University, Ping'an Street 43, Anshan 114005, China.
School of Chemistry and Life Science, Anshan Normal University, Ping'an Street 43, Anshan 114005, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Feb 5;246:118959. doi: 10.1016/j.saa.2020.118959. Epub 2020 Sep 18.
In this work, a novel phenoxazine-based fluorescent probe BPO-N was developed to detect HS. The results showed that the probe had high selectivity and sensitivity toward HS, and its detection mechanism was based the ratio between green and red fluorescence signals; its detection limit was as low as 30 nM. The fluorescent imaging experiments further showed that the probe BPO-N could successfully detect endogenous and exogenous HS in living cells. This probe can be used as a powerful tool for in-depth study of HS function in various physiological processes.
在这项工作中，开发了一种新型的基于吩恶嗪的荧光探针BPO-N用于检测硫化氢。结果表明，该探针对硫化氢具有高选择性和高灵敏度，其检测机制基于绿色和红色荧光信号的比率；其检测限低至30 nM。荧光成像实验进一步表明，探针BPO-N能够成功检测活细胞中的内源性和外源性硫化氢。该探针可作为深入研究硫化氢在各种生理过程中功能的有力工具。
