School of Computer Science, Shaanxi Normal University, Xi'an, 710119, China.
College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, China.
Comput Biol Chem. 2020 Dec;89:107374. doi: 10.1016/j.compbiolchem.2020.107374. Epub 2020 Sep 12.
In the fields of biocomputing and biomolecular, DNA molecules are applicable to be regarded as data of logical computing platform that uses elaborate logic gates to perform a variety of tasks. Graphene oxide (GO) is a type of novel nanomaterial, which brings new research focus to materials science and biosensors due to its special selectivity and excellent quenching ability. G-quadruplex as a unique DNA structure stimulates the intelligent application of DNA assembly on the strength of its exceptional binding activity. In this paper, we report a universal logic device assisted with GO and G-quadruplex under an enzyme-free condition. Integrated with the quenching ability of GO to the TAMRA (fluorophore, Carboxytetramethylrhodamine) and the enhancement of fluorescence intensity produced by the peculiar binding of G-quadruplex to the NMM (N-methylmesoporphyrin IX), a series of basic binary logic gates (AND. OR. INHIBIT. XOR) have been designed and verified through biological experiments. Given the modularity and programmability of this strategy, two advanced logic gates (half adder and half subtractor) were realized on the basis of the same work platform. The fluorescence signals generated from different input combinations possessed satisfactory results, which provided proof of feasibility. We believe that the proposed universal logical platform that operates at the nanoscale is expected to be utilized for future applications in molecular computing as well as disease diagnosis.
在生物计算和生物分子领域，DNA 分子可适当地被视为逻辑计算平台的数据，该平台使用精细的逻辑门来执行各种任务。氧化石墨烯（GO）是一种新型纳米材料，由于其特殊的选择性和优异的猝灭能力，为材料科学和生物传感器带来了新的研究焦点。G-四链体作为一种独特的 DNA 结构，凭借其出色的结合活性，激发了 DNA 组装的智能应用。在本文中，我们报告了一种在无酶条件下使用 GO 和 G-四链体辅助的通用逻辑器件。集成了 GO 对 TAMRA（荧光团，羧基四甲基罗丹明）的猝灭能力以及 G-四链体与 NMM（N-甲基甲川卟啉 IX）的特殊结合产生的荧光强度增强，通过生物实验设计并验证了一系列基本的二进制逻辑门（AND、OR、INHIBIT、XOR）。鉴于该策略的模块化和可编程性，在相同的工作平台上实现了两个高级逻辑门（半加器和半减器）。不同输入组合产生的荧光信号具有令人满意的结果，这证明了其可行性。我们相信，这种在纳米尺度上运行的通用逻辑平台有望在分子计算和疾病诊断等未来应用中得到应用。
