Xu Lijun, Hong Shanni, Shen Xiaoqiang, Zhou Lu, Wang Jine, Zhang Jianye, Pei Renjun
Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Chem Asian J. 2016 Jul 5;11(13):1892-5. doi: 10.1002/asia.201600626. Epub 2016 Jun 6.
Assembly of G-quadruplexes guided by DNA triplexes in a controlled manner is achieved for the first time. The folding of triplex sequences in acidic conditions brings two separated guanine-rich sequences together and subsequently a G-quadruplex structure is formed in the presence of K(+) . Based on this novel platform, label-free fluorescent logic gates, such as AND, INHIBIT, and NOR, are constructed with ions as input and the fluorescence of a G-quadruplex-specific fluorescent probe NMM as output.
首次实现了以可控方式由DNA三链体引导的G-四链体组装。三链体序列在酸性条件下折叠可使两个分离的富含鸟嘌呤的序列聚集在一起，随后在K(+)存在的情况下形成G-四链体结构。基于这一新型平台，构建了以离子为输入、以G-四链体特异性荧光探针NMM的荧光为输出的无标记荧光逻辑门，如与门、抑制门和或非门。
