Dhillon Jasreman
Department of Anatomic Pathology, Moffitt Cancer Center, Tampa, Florida, USA,
Department of Oncologic Sciences and Pathology, University of South Florida, Tampa, Florida, USA,
Monogr Clin Cytol. 2020;26:15-20. doi: 10.1159/000455730. Epub 2020 Sep 28.
It is important to adequately process and triage the specimen obtained from fine-needle aspirations (FNAs) of pancreatic lesions. Many echo endoscopists rely on rapid on-site evaluation (ROSE) for adequacy of FNA from solid pancreatic lesions. The role of ROSE in FNA of pancreatic lesions is discussed, as is the triage of material for making smears and cell block preparation. Different techniques of cell block preparation are briefly mentioned. Pancreatic cystic fluid obtained from pancreatic cysts is triaged differently as compared to specimens obtained from solid pancreatic lesions. An algorithmic approach to the processing of pancreatic cystic fluid for molecular and biochemical assays and cytology is discussed. Proper specimen handling is crucial to the accurate interpretation of pancreatic FNA specimens. The methods used to process a sample depend on whether the aspirated sample is solid or cystic and the type of device used for sampling. ROSE has been shown to reduce the number of inadequate specimens and to improve specimen preparation. The details of the various cytological preparation methods available are described in numerous texts. Here we focus on providing a broad overview of specimen collection and processing as it relates to pancreatic FNA, with guidance to the reader based on published and personal experiences.
对胰腺病变细针穿刺抽吸（FNA）获取的标本进行充分处理和分类至关重要。许多超声内镜医师依靠快速现场评估（ROSE）来确定胰腺实性病变FNA标本是否足够。本文讨论了ROSE在胰腺病变FNA中的作用，以及用于制作涂片和细胞块制备的材料分类。简要提及了不同的细胞块制备技术。与从胰腺实性病变获取的标本相比，从胰腺囊肿获取的胰腺囊性液体的分类方式不同。本文讨论了一种用于胰腺囊性液体分子、生化检测及细胞学检查的算法处理方法。正确的标本处理对于胰腺FNA标本的准确解读至关重要。处理样本所采用的方法取决于抽吸样本是实性还是囊性，以及用于采样的设备类型。ROSE已被证明可减少不合格标本数量并改善标本制备。众多文献中描述了各种可用的细胞学制备方法的细节。在此，我们重点基于已发表的文献及个人经验，为读者提供与胰腺FNA相关的标本采集和处理的广泛概述及指导。
