Reproduction Center, The Third Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, Henan, China.
Reproductive and Genetic Hospital, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Reprod Biol Endocrinol. 2020 Sep 25;18(1):94. doi: 10.1186/s12958-020-00651-7.
This study investigated the expression of angiopoietin-like protein 2 (ANGPTL2) in the tissues of rat models of polycystic ovary syndrome (PCOS) and its correlation with PCOS.
Six-weeks-old female specific pathogen-free rats (n = 60) were divided into blank control, PCOS model, and metformin groups (n = 20/group). After 21 days of metformin intervention, the serum sex hormones, fasting blood glucose, fasting insulin, and insulin resistance (IR) of rats in each group were measured. The mRNA levels of ANGPTL2, Foxol, and Akt in the ovarian tissues were monitored by real-time fluorescence quantitative PCR.
Compared with the control group, the levels of serum sex hormones, fasting blood glucose, fasting insulin, and IR in the model group showed significant increases, and the levels of ANGPTL2, Foxol, and Akt in the ovarian tissue also showed significant increases. Compared with the PCOS group, the serum sex hormones, fasting blood glucose, fasting insulin, and IR of rats in the metformin group were significantly decreased, and the levels of ANGPTL2, Foxol, and Akt in ovarian tissues also showed significant decreases.
These findings suggest that ANGPTL2 might participate in the development of PCOS through the PI3K/Akt signaling pathway. Metformin improves IR by reducing the expression of ANGPTL2, thus improving the endocrine environment of PCOS and might change the disease outcome.
本研究探讨了血管生成素样蛋白 2（ANGPTL2）在多囊卵巢综合征（PCOS）大鼠模型组织中的表达及其与 PCOS 的相关性。
将 6 周龄无特定病原体雌性大鼠（n=60）分为空白对照组、PCOS 模型组和二甲双胍组（每组 n=20）。二甲双胍干预 21 天后，检测各组大鼠血清性激素、空腹血糖、空腹胰岛素及胰岛素抵抗（IR）。采用实时荧光定量 PCR 检测卵巢组织中 ANGPTL2、Foxol 和 Akt 的 mRNA 水平。
与对照组相比，模型组大鼠血清性激素、空腹血糖、空腹胰岛素及 IR 水平显著升高，卵巢组织中 ANGPTL2、Foxol 和 Akt 水平也显著升高；与 PCOS 组相比，二甲双胍组大鼠血清性激素、空腹血糖、空腹胰岛素及 IR 水平显著降低，卵巢组织中 ANGPTL2、Foxol 和 Akt 水平也显著降低。
这些发现提示，ANGPTL2 可能通过 PI3K/Akt 信号通路参与 PCOS 的发生发展。二甲双胍通过降低 ANGPTL2 的表达来改善 IR，从而改善 PCOS 的内分泌环境，可能改变疾病结局。
