Dai Hongmei, Liu Fangting, Lu Jianshu, Yang Yan, Liu Pingping
Department of Reproductive Medicine, Dongying People's Hospital, Dongying, Shandong, China.
Department of Obstetrics and Gynecology, Dongying People's Hospital, Dongying, Shandong, China.
Int J Endocrinol. 2022 Jun 14;2022:2155018. doi: 10.1155/2022/2155018. eCollection 2022.
Polycystic ovary syndrome (PCOS) is a hormonal disorder that affects 5-20% of women of reproductive age. Interestingly, serum miR-124-3p and ANGPTL2 are differentially expressed in PCOS patients. Accordingly, this study set out to explore the clinical roles of serum miR-124-3p/ANGPTL2 in PCOS. Firstly, miR-124-3p/ANGPTL2 expression patterns were detected in the serum of 102 PCOS patients and 100 healthy subjects. miR-124-3p or/and ANGPTL2 diagnostic efficacy on PCOS was further analyzed, in addition to the measurement of lipid metabolism, glucose metabolism, sex hormone indexes, and inflammation levels. Correlations between serum miR-124-3p/ANGPTL2 expressions and age, BMI, Ferriman-Gallwey score, lipid metabolism, glucose metabolism, sex hormone indexes, TNF-, and IL-6 in PCOS patients were determined. The expression correlation and binding relationship of ANGPTL2 and miR-124-3p were identified. In addition, miR-124-3p was downregulated and ANGPTL2 was upregulated in the serum of obese and nonobese PCOS patients. miR-124-3p expression was found to be negatively correlated with Ferriman-Gallwey score and serum total testosterone (T), and negatively related to prolactin (PRL). ANGPTL2 expression was positively correlated with FNS and inversely linked with PRL. TNF- and IL-6 were negatively correlated with miR-124-3p, but positively correlated with ANGPTL2. Furthermore, there was a negative correlation and a targeting relationship between ANGPTL2 and miR-124-3p expression in the serum of obese and nonobese PCOS patients. Collectively, our findings indicated that miR-124-3p might target ANGPTL2 expression in obese and nonobese PCOS patients, and further underscored the diagnostic value of their combination.
多囊卵巢综合征（PCOS）是一种影响5%-20%育龄女性的激素紊乱疾病。有趣的是，血清miR-124-3p和血管生成素样蛋白2（ANGPTL2）在PCOS患者中存在差异表达。因此，本研究旨在探讨血清miR-124-3p/ANGPTL2在PCOS中的临床作用。首先，检测了102例PCOS患者和100例健康受试者血清中miR-124-3p/ANGPTL2的表达模式。除了测量脂质代谢、糖代谢、性激素指标和炎症水平外，还进一步分析了miR-124-3p或/和ANGPTL2对PCOS的诊断效能。确定了PCOS患者血清miR-124-3p/ANGPTL2表达与年龄、体重指数（BMI）、费里曼-盖尔维评分、脂质代谢、糖代谢、性激素指标、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）之间的相关性。鉴定了ANGPTL2与miR-124-3p的表达相关性和结合关系。此外，肥胖和非肥胖PCOS患者血清中miR-124-3p表达下调，ANGPTL2表达上调。发现miR-124-3p表达与费里曼-盖尔维评分和血清总睾酮（T）呈负相关，与催乳素（PRL）呈负相关。ANGPTL2表达与游离中性脂肪（FNS）呈正相关，与PRL呈负相关。TNF-α和IL-6与miR-124-3p呈负相关，但与ANGPTL2呈正相关。此外，肥胖和非肥胖PCOS患者血清中ANGPTL2与miR-124-3p表达之间存在负相关和靶向关系。总体而言，我们的研究结果表明，miR-124-3p可能在肥胖和非肥胖PCOS患者中靶向ANGPTL2表达，并进一步强调了它们联合检测的诊断价值。
