Center for Genomic Integrity, Institute for Basic Science (IBS), Ulsan, Republic of Korea.
Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
Anticancer Res. 2020 Oct;40(10):5601-5609. doi: 10.21873/anticanres.14573.
BACKGROUND/AIM: Since pathways involving LRRC17 are related to the survival of patients with various cancers, we analyzed LRRC17 as a prognostic gene in serous ovarian cancer.
Data were collected from Gene Expression Omnibus (GSE9891, GSE13876, and GSE26712) and The Cancer Genome Atlas (TCGA). We performed survival analyses using C statistics, area under the curve, survival plot with optimal cutoff level, and cox proportional regression. Zebrafish embryos were used as an in vivo model.
The prognosis of patients with high LRRC17 expression was poorer than that of patients with low LRRC17 expression. Multivariate regression analysis showed that LRRC17 expression, age, and stage were independently related with survival. Knockdown of lrrc17 reduced survival rate and delayed development in zebrafish embryos. We also found that lrrc17 is important for cell viability by protecting from p53-dependent apoptosis.
LRRC17 could be a prognostic gene in ovarian cancer as it regulates cancer cell viability through the p53 pathway.
背景/目的：由于涉及 LRRC17 的途径与各种癌症患者的生存有关，我们将 LRRC17 分析为浆液性卵巢癌的预后基因。
从基因表达综合数据库（GSE9891、GSE13876 和 GSE26712）和癌症基因组图谱（TCGA）收集数据。我们使用 C 统计、曲线下面积、具有最佳截断水平的生存图和 Cox 比例回归进行生存分析。使用斑马鱼胚胎作为体内模型。
LRRC17 高表达患者的预后比 LRRC17 低表达患者差。多变量回归分析表明，LRRC17 表达、年龄和分期与生存独立相关。lrrc17 的敲低降低了斑马鱼胚胎的存活率并延迟了其发育。我们还发现，lrrc17 通过保护 p53 依赖性细胞凋亡来维持细胞活力。
LRRC17 可能是卵巢癌的一个预后基因，因为它通过 p53 途径调节癌细胞活力。
