Centro de Biologia Molecular Severo Ochoa, CSIC-UAM, Cantoblanco, Madrid, Spain.
Nat Plants. 2020 Nov;6(11):1330-1334. doi: 10.1038/s41477-020-00770-4. Epub 2020 Sep 28.
Assessing cell proliferation dynamics is crucial to understand the spatiotemporal control of organogenesis. Here we have generated a versatile fluorescent sensor, PlaCCI (plant cell cycle indicator) on the basis of the expression of CDT1a-CFP, H3.1-mCherry and CYCB1;1-YFP, that identifies cell cycle phases in Arabidopsis thaliana. This tool works in a variety of organs, and all markers and the antibiotic resistance are expressed from a single cassette, facilitating the selection in mutant backgrounds. We also show the robustness of PlaCCI line in live-imaging experiments to follow and quantify cell cycle phase progression.
评估细胞增殖动态对于理解器官发生的时空控制至关重要。在这里，我们基于 CDT1a-CFP、H3.1-mCherry 和 CYCB1;1-YFP 的表达，生成了一种通用的荧光传感器 PlaCCI（植物细胞周期指示剂），可鉴定拟南芥中的细胞周期阶段。该工具可在多种器官中使用，并且所有标记物和抗生素抗性均由单个盒表达，这有助于在突变背景中进行选择。我们还展示了 PlaCCI 系在活体成像实验中用于跟踪和定量细胞周期阶段进展的稳健性。
