Schneider M D, Perryman M B, Payne P A, Spizz G, Roberts R, Olson E N
Mol Cell Biol. 1987 May;7(5):1973-7. doi: 10.1128/mcb.7.5.1973-1977.1987.
Myogenic differentiation is obligatorily coupled to withdrawal of myoblasts from the cell cycle and is inhibited by specific polypeptide growth factors. To investigate the potential involvement of c-myc in the control of myogenesis, the BC3H1 muscle cell line was stably transfected with a simian virus 40 promoter:c-myc chimeric gene. In quiescent cells in 0.5% serum, the exogenous c-myc gene was expressed at a level more than threefold greater than the level of endogenous c-myc in undifferentiated, proliferating cells of the parental line in 20% serum. The transfected myc gene partially inhibited the expression of both muscle creatine kinase and the nicotinic acetylcholine receptor, but was not sufficient to prevent the induction of these muscle differentiation products upon mitogen withdrawal.
成肌分化必然与成肌细胞退出细胞周期相关联，并受到特定多肽生长因子的抑制。为了研究c-myc在成肌控制中的潜在作用，用猿猴病毒40启动子：c-myc嵌合基因稳定转染了BC3H1肌肉细胞系。在含0.5%血清的静止细胞中，外源性c-myc基因的表达水平比在含20%血清的亲代系未分化、增殖细胞中内源性c-myc的水平高出三倍以上。转染的myc基因部分抑制了肌肉肌酸激酶和烟碱型乙酰胆碱受体的表达，但不足以阻止在有丝分裂原撤除后这些肌肉分化产物的诱导。
