Denis N, Blanc S, Leibovitch M P, Nicolaiew N, Dautry F, Raymondjean M, Kruh J, Kitzis A
Institut de Pathologie Moléculaire, Faculté de Médicine Cochin-Port-Royal, Paris, France.
Exp Cell Res. 1987 Sep;172(1):212-7. doi: 10.1016/0014-4827(87)90107-8.
The role of c-myc oncogene expression in myogenic differentiation has been established by transfecting rat myoblasts of the L6 cell line with plasmid pMT-myc, in which the c-myc coding sequences were under the control of the metallothionein I promoter. We observed that the constitutive expression of the exogenous c-myc gene inhibits muscular differentiation. A diminution of the endogenous c-myc gene expression occurs within the first 24 h after the transfer of the cells to a differentiating medium. This early decrease of c-myc expression is required for cell differentiation to occur. We have also observed that exogenous myc gene expression has no effect on endogenous myc expression.
通过用质粒pMT-myc转染L6细胞系的大鼠成肌细胞，已确定c-myc癌基因表达在肌源性分化中的作用，其中c-myc编码序列受金属硫蛋白I启动子的控制。我们观察到外源性c-myc基因的组成型表达抑制肌肉分化。将细胞转移到分化培养基后的最初24小时内，内源性c-myc基因表达会减少。c-myc表达的这种早期下降是细胞分化发生所必需的。我们还观察到外源性myc基因表达对内源性myc表达没有影响。
