使用多维双螺旋（MDDS）惯性微流控技术的全自动且可现场部署的血液白细胞分离平台。

Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics.

作者信息

Jeon Hyungkook, Jundi Bakr, Choi Kyungyong, Ryu Hyunryul, Levy Bruce D, Lim Geunbae, Han Jongyoon

机构信息

Research Laboratory of Electronics, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.

Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA.

出版信息

Lab Chip. 2020 Sep 29;20(19):3612-3624. doi: 10.1039/d0lc00675k.

DOI:10.1039/d0lc00675k

PMID:32990714

Abstract

A fully-automated and portable leukocyte separation platform was developed based on a new type of inertial microfluidic device, multi-dimensional double spiral (MDDS) device, as an alternative to centrifugation. By combining key innovations in inertial microfluidic device designs and check-valve-based recirculation processes, highly purified and concentrated WBCs (up to >99.99% RBC removal, ∼80% WBC recovery, >85% WBC purity, and ∼12-fold concentrated WBCs compared to the input sample) were achieved in less than 5 minutes, with high reliability and repeatability (coefficient of variation, CV < 5%). Using this, one can harvest up to 0.4 million of intact WBCs from 50 μL of human peripheral blood (50 μL), without any cell damage or phenotypic changes in a fully-automated operation. Alternatively, hand-powered operation is demonstrated with comparable separation efficiency and speed, which eliminates the need for electricity altogether for truly field-friendly sample preparation. The proposed platform is therefore highly deployable for various point-of-care applications, including bedside assessment of the host immune response and blood sample processing in resource-limited environments.

摘要

基于一种新型惯性微流控装置——多维双螺旋（MDDS）装置，开发了一种全自动便携式白细胞分离平台，以替代离心法。通过结合惯性微流控装置设计和基于止回阀的再循环过程中的关键创新，在不到5分钟的时间内实现了高度纯化和浓缩的白细胞（红细胞去除率高达>99.99%，白细胞回收率约80%，白细胞纯度>85%，与输入样本相比白细胞浓缩约12倍），具有高可靠性和可重复性（变异系数，CV<5%）。使用该平台，在全自动操作中，从50μL人外周血中可收获多达40万个完整白细胞，且无任何细胞损伤或表型变化。此外，还展示了手动操作，其分离效率和速度相当，完全无需电力，实现了真正便于现场使用的样本制备。因此，该平台非常适合用于各种即时护理应用，包括在资源有限环境中对宿主免疫反应的床边评估和血样处理。