微泵集成白细胞分离平台用于慢性肉芽肿病检测。

Micropump integrated white blood cell separation platform for detection of chronic granulomatous disease.

机构信息

Department of Mechanical Engineering, BITS-Pilani, K K Birla Goa Campus, Sankval, Goa, 403726, India.

Department of Chemistry, BITS-Pilani, K K Birla Goa Campus, Sankval, Goa, 403726, India.

出版信息

Mikrochim Acta. 2024 May 3;191(5):295. doi: 10.1007/s00604-024-06372-7.

DOI:10.1007/s00604-024-06372-7

PMID:38700804

Abstract

White blood cells (WBCs) are robust defenders during antigenic challenges and prime immune cell functioning indicators. High-purity WBC separation is vital for various clinical assays and disease diagnosis. Red blood cells (RBCs) are a major hindrance in WBC separation, constituting 1000 times the WBC population. The study showcases a low-cost micropump integrated microfluidic platform to provide highly purified WBCs for point-of-care testing. An integrated user-friendly microfluidic platform was designed to separate WBCs from finger-prick blood (⁓5 μL), employing an inertial focusing technique. We achieved an efficient WBC separation with 86% WBC purity and 99.99% RBC removal rate in less than 1 min. In addition, the microdevice allows lab-on-chip colorimetric evaluation of chronic granulomatous disease (CGD), a rare genetic disorder affecting globally. The assay duration, straight from separation to disease detection, requires only 20 min. Hence, the proposed microfluidic platform can further be implemented to streamline various clinical procedures involving WBCs in healthcare industries.

摘要

白细胞（WBCs）是抗原挑战期间强大的防御者，也是免疫细胞功能的主要指标。高纯度的 WBC 分离对于各种临床检测和疾病诊断至关重要。红细胞（RBCs）是 WBC 分离的主要障碍，其数量是 WBC 的 1000 倍。本研究展示了一种低成本的微泵集成微流控平台，用于为即时检测提供高度纯化的 WBC。设计了一个集成的用户友好型微流控平台，采用惯性聚焦技术从手指采血（⁓5 μL）中分离 WBC。我们在不到 1 分钟的时间内实现了高效的 WBC 分离，WBC 纯度达到 86%，RBC 去除率达到 99.99%。此外，该微器件允许对慢性肉芽肿病（CGD）进行片上比色评估，这是一种影响全球的罕见遗传疾病。从分离到疾病检测的整个检测过程仅需 20 分钟。因此，所提出的微流控平台可以进一步应用于简化医疗保健行业中涉及 WBC 的各种临床程序。