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枯草芽孢杆菌在耦合和非耦合钙源与碳源中实现方解石结晶的新方法。

Novel method to achieve crystallinity of calcite by Bacillus subtilis in coupled and non-coupled calcium-carbon sources.

作者信息

Ferral-Pérez Héctor, Galicia-García Mónica, Alvarado-Tenorio Bonifacio, Izaguirre-Pompa Aldo, Aguirre-Ramírez Marisela

机构信息

Laboratorio de Biología Celular y Molecular, Departamento de Ciencias Químico-Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, 32310, Cd. Juárez, CHIH, Mexico.

Laboratorio de Electroquímica, Departamento de Ciencias Químico-Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, 32310, Cd. Juárez, CHIH, Mexico.

出版信息

AMB Express. 2020 Sep 29;10(1):174. doi: 10.1186/s13568-020-01111-6.

DOI:10.1186/s13568-020-01111-6
PMID:32990816
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7524977/
Abstract

Bacteria mineralization is a promising biotechnological approach to apply in biomaterials development. In this investigation, we demonstrate that Bacillus subtilis 168 induces and influences CaCO composites precipitation. Crystals were formed in calcium-carbon non-coupled (glycerol + CaCl, GLY; or glucose + CaCl, GLC) and coupled (calcium lactate, LAC; or calcium acetate, ACE) agar-sources, only maintaining the same Ca concentration. The mineralized colonies showed variations in morphology, size, and crystallinity form properties. The crystals presented spherulitic growth in all conditions, and botryoidal shapes in GLC one. Birefringence and diffraction patterns confirmed that all biogenic carbonate crystals (BCC) were organized as calcite. The CaCO in BCC was organized as calcite, amorphous calcium carbon (ACC) and organic matter (OM) of biofilm; all of them with relative abundance related to bacteria growth condition. BCC-GLY presented greatest OM composition, while BCC-ACE highest CaCO content. Nucleation mechanism and OM content impacted in BCC crystallinity.

摘要

细菌矿化是一种很有前景的生物技术方法，可应用于生物材料的开发。在本研究中，我们证明枯草芽孢杆菌168能诱导并影响碳酸钙复合材料的沉淀。晶体在钙碳非耦合（甘油+氯化钙，GLY；或葡萄糖+氯化钙，GLC）和耦合（乳酸钙，LAC；或醋酸钙，ACE）琼脂源中形成，仅保持相同的钙浓度。矿化菌落的形态、大小和结晶度形式特性存在差异。晶体在所有条件下均呈现球晶生长，在GLC条件下呈现葡萄状形态。双折射和衍射图谱证实所有生物源碳酸盐晶体（BCC）均为方解石结构。BCC中的碳酸钙由方解石、无定形碳酸钙（ACC）和生物膜的有机物（OM）组成；它们的相对丰度均与细菌生长条件有关。BCC-GLY的OM组成最大，而BCC-ACE的碳酸钙含量最高。成核机制和OM含量影响BCC的结晶度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef2d/7524977/e7ac973585f2/13568_2020_1111_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef2d/7524977/dde257b2c5b5/13568_2020_1111_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef2d/7524977/8a3de36f87c1/13568_2020_1111_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef2d/7524977/8e6963257dc5/13568_2020_1111_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef2d/7524977/b7ce038cc023/13568_2020_1111_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef2d/7524977/06db2a589661/13568_2020_1111_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef2d/7524977/e7ac973585f2/13568_2020_1111_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef2d/7524977/dde257b2c5b5/13568_2020_1111_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef2d/7524977/8a3de36f87c1/13568_2020_1111_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef2d/7524977/8e6963257dc5/13568_2020_1111_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef2d/7524977/b7ce038cc023/13568_2020_1111_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef2d/7524977/06db2a589661/13568_2020_1111_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef2d/7524977/e7ac973585f2/13568_2020_1111_Fig6_HTML.jpg

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Appl Environ Microbiol. 2020 Mar 18;86(7). doi: 10.1128/AEM.02739-19.
3
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4
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5
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J Vis Exp. 2019 May 14(147). doi: 10.3791/59638.
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