Wnt 反应性连接上皮的形成和再生。
Formation and regeneration of a Wnt-responsive junctional epithelium.
机构信息
Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Palo Alto, CA, USA.
State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
出版信息
J Clin Periodontol. 2020 Dec;47(12):1476-1484. doi: 10.1111/jcpe.13371. Epub 2020 Nov 9.
AIM
To identify the molecular mechanisms mediating the persistent defensive functions of the self-renewing junctional epithelium (JE).
MATERIALS AND METHODS
Two strains of Wnt reporter mice, Axin2 ;R26R and Axin2 , were employed, along with three clinically relevant experimental scenarios where the function of the JE is disrupted: after tooth extraction, after a partial gingivectomy, and after a complete circumferential gingivectomy.
RESULTS
Using transgenic Wnt reporter strains of mice, we established the JE is a Wnt-responsive epithelium beginning at the time of its formation and that it maintains this status into adulthood. After tooth extraction, progeny of the initial Wnt-responsive JE population directly contributed to healing and ultimately adopted an oral epithelium (OE) phenotype. In the traditional partial gingivectomy model, the JE completely regenerated and did so via progeny of the original Wnt-responsive population. However, following circumferential gingivectomy, the OE was incapable of re-establishing a functional JE.
CONCLUSIONS
A Wnt-responsive niche at the interface between tooth and oral epithelia is required for a functional JE.
目的
确定介导自我更新的连接上皮（JE）持久防御功能的分子机制。
材料和方法
使用了两种 Wnt 报告基因小鼠品系，Axin2 ; R26R 和 Axin2 ，以及三种临床上相关的 JE 功能被破坏的实验情况：拔牙后、部分牙龈切除术和全周牙龈切除术。
结果
利用小鼠的转基因 Wnt 报告基因株，我们证实 JE 是一种 Wnt 反应性上皮，从其形成开始就具有这种状态，并在成年期保持这种状态。拔牙后，初始 Wnt 反应性 JE 群体的后代直接参与了愈合，并最终采用了口腔上皮（OE）表型。在传统的部分牙龈切除术模型中，JE 完全再生，并且通过原始 Wnt 反应性群体的后代来实现。然而，在全周牙龈切除术后，OE 无法重新建立功能性 JE。
结论
在牙齿和口腔上皮之间的界面处需要一个 Wnt 反应性龛位才能维持 JE 的功能。
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