Wnt 响应性 PDL 群体促进拔牙窝愈合。
A Wnt-Responsive PDL Population Effectuates Extraction Socket Healing.
机构信息
1 Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford University, Stanford, CA, USA.
2 State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
出版信息
J Dent Res. 2018 Jul;97(7):803-809. doi: 10.1177/0022034518755719. Epub 2018 Feb 8.
Stem cells residing in the periodontal ligament (PDL) support the homeostasis of the periodontium, but their in vivo identity, source(s), and function(s) remain poorly understood. Here, using a lineage-tracing mouse strain, we identified a quiescent Wnt-responsive population in the PDL that became activated in response to tooth extraction. The Wnt-responsive population expanded by proliferation, then migrated from the PDL remnants that remained attached to bundle bone, into the socket. Once there, the Wnt-responsive progeny upregulated osteogenic protein expression, differentiated into osteoblasts, and generated the new bone that healed the socket. Using a liposomal WNT3A protein therapeutic, we showed that a single application at the time of extraction was sufficient to accelerate extraction socket healing 2-fold. Collectively, these data identify a new stem cell population in the intact periodontium that is directly responsible for alveolar bone healing after tooth removal.
牙周膜（PDL）中的干细胞支持牙周组织的稳态，但它们的体内身份、来源和功能仍知之甚少。在这里，我们使用谱系追踪小鼠品系，鉴定出牙周膜中存在一个静止的 Wnt 反应性群体，该群体在牙齿拔除后被激活。Wnt 反应性群体通过增殖而扩大，然后从附着在束骨上的剩余牙周膜中迁移，进入牙槽骨。一旦进入牙槽骨，Wnt 反应性祖细胞上调成骨蛋白表达，分化为成骨细胞，并产生新的骨组织来修复牙槽骨。使用脂质体 WNT3A 蛋白治疗，我们表明，在拔牙时单次应用就足以将拔牙窝愈合速度提高 2 倍。总的来说，这些数据鉴定出完整牙周组织中的一种新的干细胞群体，该群体直接负责牙齿去除后牙槽骨的愈合。
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